Nondestructive testing of defects in adhesive joints

pattern appears to be a little diffused due to smaller particle sizes, but three diffraction rings are
clearly visible and they can be indexed to the face-centered cubic structure of silver as follows.
The strongest ring and the one closest to the center is probably a combination of the (111) and
(200) reflections. The second ring is likely the (222) reflection whereas the outermost and the
weakest third ring are either the (420) and/or the (422) reflections. Almost similar results have
also been reported elsewhere [18]. The size distributions were obtained by measuring the
diameters of 35 particles in an arbitrarily chosen area of TEM image [see Fig. 4 (B)]. As can be
seen, nearly 40% particles have an average diameter of 26 nm and moreover, the distribution
curve appears to be almost symmetrical with all the nanoparticles falling within the narrow range
of 12 to 42 nm.
Antibacterial property of nanosilver loaded GFP
Finally, we carried out antibacterial test for the prepared silver nanoparticles loaded
GFP against E.coli. Fig.5 (A) clearly demonstrates dense population of bacterial colonies on the
whole surface of the grafted filter paper which is simply due to absence of silver nanoparticles
in the sample. However, in Fig 5 (B) a clear zone of inhibition can be seen on the whole surface
of the nanosilver loaded grafted filter paper. This may simply be explained on the basis of the
fact that as the filter paper contains silver nanoparticles, the colonization of bacteria is greatly
inhibited on the nano Ag-loaded filter paper. Therefore it may be concluded that silver
nanoparticles loaded grafted filter paper possesses strong antibacterial property.
4. Conclusion
From the above study it may be concluded that grafting of acrylamide onto filter
paper, followed by incorporation of silver nanoparticles results in development of a
novel biomaterial which demonstrates fair biocidal action against E.coli, and it can be used as
an antibacterial packaging material to prevent food stuff from bacterial infection. As the
proposed method does not involve use of toxic organic solvents or harsh conditions like high
temperature, the strategy can be applied for the manufacture of antibacterial food packaging
material.
Acknowledgement
I would like to owe my whole hearted gratitude to my supervisor Dr. S.K. Bajpai, Prof. of
Chemistry, Govt. Model Science College, Jabalpur (M.P.) for his kind guidance.
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