2009_Book_FoodChemistry

4.5 References 337
Table 4.29. Cellulases
EC No. Name Synonym Reaction
3.2.1.4 Cellulase C x factor Endohydrolysis of
CMCase a , 1,4-β-D-glucosidic bonds
endo-1,4-βglucanase
3.2.1.91 Cellulose C 1 factor Exohydrolysis of
1,4-β-cellobiosidase avicellase 1,4-β-D-glucosidic bonds with
formation of cellobiose from
cellulose or 1,4-β-glucooligosaccharides.
The attack proceeds
from the nonreducing end.
3.2.1.21 β-Glucosidase Cellobiase Hydrolysis of terminal β-Damygdalase
glucose residues in β-glucans
a CMC: carboxymethylcellulose; the enzyme activity can be measured via the decrease in
viscosity of a CMC solution.
the insoluble fibers remain in the residue. The
water soluble fibers in the supernatant are isolated
by precipitation with ethanol, ultrafiltration
or dialysis. The protein and mineral matter still
remaining with the soluble and insoluble dietary
fibers is deducted with the help of correction
factors.
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