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2009_Book_FoodChemistry

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1.4 Proteins 45

Fig. 1.11. Subtilisin BPN; peptide bonds hydrolyzed by trypsin (T), chymotrypsin (C), and pepsin (P)

segment from the hydrolysis of protein by

trypsin has lysine as its C-terminal amino acid.

It is attached to the carrier with p-phenylenediisothiocyanate

through the α- and ε-amino

groups. Mild acidic treatment of the carrier

under conditions of the Edman degradation splits

the first peptide bond. The Edman procedure

is then performed on the shortened peptide

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