2009_Book_FoodChemistry

752 16 Legumes
Table 16.8. Amino acid sequences of subunits a of the 7 S globulins, 1) β-conglycinin α ′ (Glycine max), 2) phaseolin
(Phaseolus vulgaris) and 3) vicilin (Pisum sativum)
1 10 20 30 40 50
1 b – – – – DEDEEQDKES QES EG SES Q REP R R HKNKNP FHF NS KR– F QTLF KNQ
2 A TS LREEEE––––S QD––– ––– – – – – – – – – – – NP FYF NS DNS WNTLF KNQ
3 – –– ––––––––R–– ––––– –SDPQ––– – –––––NPFI FKSNK–F QTLFENE
51 60 70 80 90 100
1 Y GH VRVLQRFNKRS QQLQN LRD Y RI LE F NSKP NTLLLP HHAD A DYLI VI L
2 Y GHI RVLQRFDQQS KRLQN LEDYRLVE F RSKPETLLLPQQAD AELLLVVR
3 N GH I RLLQKFDQRS KI F EN–QNY R LLEY K S KP HTI FLP QHTDA D YI LVVL
101 110 120 130 140 150
1 N GT AI LTLVNNDDRDSY–N LQS GDA–L – ––––RVPAGTTFYV VNPDNDEN
2 S GS AI LVLVKP DDR REYF F LTQ G DNP I F S DNQKI P––––––– – –––––––
3 S GKAI LTVLKPDDRNSF–N –ERGDT–I – ––––KLP––––––– – –––––––
151 160 170 180 190 200
1 L RMI AGTTF YVVNP DNDEN LRMI TLAI P VNKP ERFESFFLSS T QAQQS YL
2 – – – – AGTI F YLVNP DP KED LRI I QLAMP VNNP Q– I HEF F LS S T EAQQS YL
3 – – – – AGTI AYLVNR DDNEE LRV L DLAI P VNRP GQLQS F LLS G N QNQQNYL
201 210 220 230 240 250
1 Q GFSKNI LEASYDTKFEEI NKVLFGREEGQQ–Q–––G–––EE R––––LQE
2 Q EFSKHI LEASFNSKFEEI NRVLF–EEEGQQ––––––––––– – –EEGQQE
3 S GF SKNI LEASFNT DYEEI EKVL L–EE HEKETQHRRSLKD–K R –QQSQEE
251 260 270 280 290 300
1 S VI VEI S KKQI REL S KHAK SS S R KTI S S EDKP F NLGS RDPI Y S NKLGKLF
2 G VI VNI DS EQI EEL S KHAK SS S R KS HS – – – KQ– D– –––NTI – GNEFGNLT
3 N VI VKLS RGQI EEL S KNAK STS K KS VS S ESEP F NLRS RGPI Y S NEF GKF F
301 310 320 330 340 350
1 E I T – QRN– P QLRDL DVF LS VVD MNEGA L F LP HF NSKAI VVLV I NEGEANI
2 E RT – D– N– – – – S – L NVLI S SI E MKEGA L F VP HYYSKAI VI LV V NEGEAHV
3 E I T P EKN– P QLQDL DI F VN SVE I KEGS L LLP HYNSRAI VI VT V NEGKGDF
351 360 370 380 390 400
1 E LVGI K–––––––– –––EQ QQRQQQ–– – EEQ––P–––––LEV RKYRAELS
2 E LVGPK–––––––– –––GN ––– – KE–– – –––––––––T–LEF ESYRAELS
3 E LVGQR–––––––– ––NEN QQEQRKEDDEEEEQGEEEI NKQV QNYKAKLS
401 410 420 430 440 450
1 E QD I F VI P AGYP VMVNATS DLN F – F – A F G– ––––INAENNQR NFLAGSKD
2 K DDVFVI PAAYPVAI KATS NVNF––TGFG–––––INANNNNR N LLAGKTD
3 S GDVFVI PAGHPVALKASS NLDL–L–GFG–––––INAENNQR NFLAGDED
451 460 470 480 490 500
1 N VI SQI PSQV––QE–––LA FPRSAKDI E NLI KS Q– S ES YFVD A ––––QPQ
2 X VI S S I GRALDGKD VLGLT FS G S GEEV MKLI NKQ– S GS YFVD G HHHQQEQ
3 N VI S QVQRP V– – KE –––LA FPGSAQEVDRILENQ–KQSHFAD A––––QPQ
501 510 520 530
1 Q KE E– GN– – – – – – K GRKGP LS S I LRAF – Y
2 Q K– – – GS HQQEQQK GRKG– – – – – – – AF V Y
3 Q RE–RGS––RE–TRDR––– LSSV
a α/β cleavage site: 239/240, β/γ cleavage site: 376/377; – – space to maximize homology.
b In order to show the homology clearly, the N-terminal of β-conglycin was omitted in the table. It consists of the
following sequence:
VEEEEECEEGQIPRPRPQHPERERQQHGEKEEDEGEQPRPFPFPRPRQPHQ
EEEHEQKEEHEWHRKEEKHGGKGSEEEQDEREHPRPHQPHQKEEEKHEW
HKQEQKHQGKESEEEEEDQ