4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 108 Klinička citologija - Usmena predavanja EVALUATION OF THE HPV L1 CAPSID PROTEIN IN PROGNOSIS OF MILD AND MODERATE DYSPLASIA OF THE CERVIX UTERI Štemberger-Papić S1 , Vrdoljak-Mozetič D1 , Verša Ostojić D1 , Rubeša-Mihaljević R1 , Markanjević T1 , Rešetar R1 , Manestar M2 1 Department of Gynecological Cytology, University Department of Gynecology and Obstetrics, University Hospital Centre Rijeka, Croatia 2 Department of Gynaecology, University Department of Gynaecology and Obstetrics, University Hospital Centre Rijeka, Croatia Objective: To determine weather HPV L1 immunoreactivity could help us to discriminate between regresing and persistent/progressing mild and moderate cervical dysplasia in cytological specimens. Materials and Methods: We immunostained archival Pap smears from 114 hr HPV DNA positive squamous lesions with mild or moderate dysplasia. The staining results were correlated with follow-up smears for lesions which are going to remission or for persistent lesions with mild dysplasia. Progressive or persistent lesions with moderate dysplasia were correlated with histologic verification on Large Loop Excision of the Transformation Zone (LLETZ) or conization. Results: Immunochemical analyses with L1 antibody revealed positively stained nuclei of squamous epithelial cells in 56 of 114 smears (49,1%). Regression (negativisation of the Pap smear for 24 months or longer) was noticed in 31 of 56 ( 55,4% ) L1-positive cases and in 20 of 58 (34,5%) L1-negative cases. Persistent disease occured in 13 (23,2%) L1-positive cases and in 14 (24,1%) L1-negative cases. Progressive disease occured in 12 (21,4%) L1-positive cases and in 24 (41,4%) L1-negative cases. The difference in the clinical course between the L1-positive and L1-negative patients was statistically significant (p=0,025). Also, the difference in the clinical course of the L1-negative staining in the under-30 and over-30 years age group was statistically significant (p=0,04). Conclusion: Our data confirm that immunostaining for HPV L1 capsid protein could offer prognostic information about mild and moderate intraepithelial cervical squamous lesions. snjezana.stemberger@ri.t-com.hr
Clinical Cytology - Oral Presentations BILIARY BRUSH CYTOLOGY FOR THE DIAGNOSIS OF MALIGNANCY: A SINGLE CENTER EXPERIENCE Štoos-Veic T1 , Bilic B2 , Kaic G1 , Trutin Ostovic K1 , Babic Z2 , Kujundzic M2 1 Department of Cytology and Flow Cytometry, Dubrava University Hospital, Zagreb, Croatia 2 Department of Gastroenterology, Dubrava University Hospital, Zagreb, Croatia Brush cytology during the endoscopic retrograde cholangiopancreatography (ERCP) is the most commonly used method for obtaining tissue confirmation of the nature of biliary strictures. Its specificity is remarkably high but reported sensitivities for the diagnosis of malignancy are low. Aim of our study was to assess sensitivity and specificity of biliary brush cytology in our institution, to find out main causes of false negative diagnoses and to confirm impression that the team approach has impact on sensitivity. Materials and methods: Out of total of 201brushings, 143 specimens with available definitive diagnosis were analyzed. Gold standard for diagnosis was definitive surgical histology or adequate clinical follow up for minimum of six month. Direct smears made by cytotechnician at the endoscopy room, and stained according to Papanicolaou and May-Grünwald Giemsa (MGG), were examined for well-recognized features of malignancy on conventional smears as a part of diagnostic routine. Cytologic diagnoses were benign, atypical/reactive, suspicious for malignancy and malignant. Out of 143specimens, 36 (25%) had malignant cytologic diagnosis and 91(63.6%) were benign, 3 were atypical/reactive and 13 suspicious for malignancy with 20 “false-negative” cases. When specimens with atypical and suspicious cytology were excluded from data analysis sensitivity was 64% and specificity was 100% and when suspicious findings were taken into account as true positives, sensitivity rose to 71%. Conclusion: We find that biliary brush cytology, although mainly depending on the skill of endoscopist, as well as the experience of the cytologist, is a valuable method for obtaining accurate tissue diagnosis of biliary strictures, thus solving eternal diagnostic dilemma: benign or malignant. tveic@kbd.hr 109 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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