4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije THE ROLE OF CYTOTECHNOLOGIST IN CYTOANALYSIS OF SYNOVIAL FLUIDS Bezdrob S, Aleksandrov C, Cvrk B, Čurin S, Ljevar I, Krupec A, Čurin D, Knežević A, Perica B, Ćaleta B, Trutin Ostović K Dubrava University Hospital, Zagreb, Croatia Aim: To point out possible mistakes in procedures in cytoanalysis of synovial fluids processed by cytotechnologists. Methods: Cytoanalysis is performed by two steps: urgent and standard. The urgent examination consists of macroscopic analysis (determination of volume, colour, clarity, viscosity and mucin clot test), counting of total count of nuclear cells in Burker-Turk counting chamber, native microscopic analysis for crystals and analysis of neutrophil granulocyte percentage in cytospin sediments stained by Hemacolor. All these procedures except the last two are performed only by cytotechnologists. They are done at least by two persons separately one from another because of subjective analysis. Standard procedure consists of microscopic examination of sediments of synovial fluids stained by Papenheim, Papanicolaou and cytochemistry of acid phosphatase done by cytotechnologists (semiquantitative analysis) and by cytologist (qualitative and semiquantitative analysis) separately. Results: We have analysed 115 synovial fluids, 81 patients with inflammation (type I and II) and 34 patients with degenerative illnesses and injuries (type 3). Conclusion: Cytotechnologists play very important role in cytological diagnosis of synovial fluids and therefore well education is necessary.Citotehnolozi/ CytotechnologistsComputationalORAL samirbezdrob@yahoo.com 172 Citotehnologija - Usmena predavanja
Cytotechnology - Oral Presentations MODIFICATION OF MGG STAINING METHOD Fumić G, Naglić G, Meandžija R, Štemberger C, Seili-Bekafigo I. Department of Cytology, Department of Internat Medicine, Clinical Hospital Center Rijeka, Croatia Aim: May-Gruenwald.Giemsa staining method is a standard procedure in fine-needle aspiration cytology (FNA). There are some commercial kits for rapid staining available, but modified MGG technique could be used for intraoperative analysis, and for rapid on-site examination of smears. If, due to some errors in staining procedure, slides are over-stained with MGG, there is a simple procedure for partial destaining. Materials and methods: For intraoperative examinations we used the modified protocol: shortened staining time, and increased concentration of Giemsa (G). For rapid on-site examination, minimum of equipment needed was: a microscope, May-Gruenwald (MG) staining solution, tap water and distilled water, and a shallow dish with a rack. Air-dried smears were stained with MG. Afterwards we continued the staining procedure with Giemsa stain in the laboratory. Overstained slides were treated by immersing 3 times in 80% ethanol. Results: Rapid staining with MGG in 5’ 30’’ for liquid specimens, or in 4’ 30’’ for smears, provided preparations good enough for cytologic analysis during the operation. Staining with MG only, enabled us to evaluate the quality of specimen while patient is still available for repeating FNA, without losing those slides for definitive examination. Immersing over-stained MGG smears in 80% ethanol, made them suitable for analysis. Conclusion: Using standard stains, MG and G, it is possible to perform rapid intraoperative staining and rapid on-site evaluation of FNA specimens, without additional costs. Slides accidentally overstained can easily be partially destained with 80% ethanol. gordana.fumic@ri.t-com.hr 173 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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