4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 64 Klinička citologija - Plenarna i pozvana predavanja MORPHOMETRIC AND DNA IMAGE ANALYSIS OF BRONCHOALVEOLAR LAVAGE FLUID MACROPHAGES NUCLEI IN INTERSTITIAL LUNG DISEASES WITH LYMPHO- CYTIC ALVEOLITIS Smojver-Ježek S1 , Peroš-Golubičić T2 , Tekavec-Trkanjec J2 , Alilović M2 , Vrabec-Branica B1 , Juroš Z1 , Mažuranic I3 University Hospital for Lung Diseases “Jordanovac”, Zagreb, Croatia 1 Department of Cytology 2 Department of Pneumology 3 Department of Radiology Lymphocytic alveolitis is a characteristic of diverse interstitial lung diseases (ILD-s), but macrophages are often more numerous cell population in bronchoalveolar lavage fluid (BALF). Aim of this study is to analyze morphometric characteristics of macrophages nuclei in BALF in patients with ILD-s and to detect possible differences allowing distinguishing sarcoidosis from other lymphocytic alveolitis ILD-s. Patients and methods. Thirty-one patient with interstitial lung disease who had lymphocytic alveolitis in BALF cell count (17 sarcoidosis and 14 other ILD-s) and nine controls were included in the study. The following patients data were numbered: age, lymphocyte percentage and CD4/CD8 ratio in BALF. Investigated morphometric parameters of macrophages nuclei were: area, outline, maximal radius, minimal radius, length, breadth, form factor (FF), elongation factor (EF) and DNA image cytometry ploidy status determined with Van Velthoven method. Results. Predicted classifications in classification matrix (forward step-wise method in multivariate discriminant function analysis) based on macrophages nuclei length mean, minimum and maximum, breadth SD, FF mean and lymphocyte % were 100% (9/9) correct for control group, 88.235% (15/17) correct for sarcoidosis, and 92.857% (13/14) correct for other lymphocytic alveolitis ILD group. In total, 92.5% (37/40) of the examinees were correctly classified in particular group upon the observed variables. ssmojver@pbf.hr
Clinical Cytology - Plenary and Invited Lectures CYTOMORPHOLOGY OF SELECTED SUBTYPES OF ACUTE LEUKEMIA (AL) Sučić M1 , Marković-Glamočak M1 , Ries S1 , Gjadrov-Kuveždić K1 , Antulov J1 , Fabijanić I1 , Vrbanus LJ1 , Ilić I1 , Dotlić S1 , Čačić M1 , Zadro R2 , Mrsić S2 , Franić Šimić I2 , Batinić D2 , Dubravčić K2 , Serventi-Seiwerth R3 , Sertić D3 , Mikulić M3 , Mrsić M3 , Nemet D3 , Labar B3 1 Zagreb University Clinical Hospital Center and School of Medicine, Clinical Department of Pathology and Cytology, Zagreb, Croatia 2 Zagreb University Clinical Hospital Center and School of Medicine, 2Clinical Institute of Laboratory Diagnosis, Zagreb, Croatia 3Zagreb University Clinical Hospital Center and School of Medicine 3Department of Hematology, Zagreb, Croatia According to WHO classification of tumors of hematopoietic and lymphoid tissues, the major subgroups of acute myeloid leukemia (AML) and related precursor neoplasms are the following: AML with recurrent genetic abnormalities, AML with myelodisplasia, therapy-related myeloid neoplasms, AML-(NOS), myeloid sarcoma, myeloid proliferations related to Down syndrome and blastic plasmocytoid dendritic cell neoplasm. Acute lymphoblastic leukemia is classified together with B and T lymphoblastic lymphoma in a group of precursor lymphoid neoplasms. Mixed lineage acute leukemia (MPAL) and acute undifferentiated leukemia (AUL) are subtypes of AL of ambiguous lineage. The aim of the study was to analyze cytomorphology of selected AML and MPAL subtypes. Patients and methods: In the study are included AL patients treated at Zagreb University Hospital Center. Bone marrow and peripheral blood of AL patients were analyzed after Pappenheim and cytochemical staining and classified according FAB and WHOclassification. Results: Therapy related AML was diagnosed in two of AL patients: one had secondary AML-M4 after five years of initial diagnosis and treatment for B-NHL, and another had AML-M2 after diagnosis of breast carcinoma and melanoma. In one patient with AML- M8 the blasts were cytomorphologically undifferentiated with sparse mature basophiles, whereas in one AML-M1 patient blasts at relapse expressed lymphoid cytomorphology pointing to lineage switch (phenomenon of MPAL). Conclusion: Cytological diagnosis of AL subtypes together with multidisciplinary diagnostic approach is essential in initial diagnosis and continuous follow-up of patients with AL, especially for secondary AL, and some other subtypes of AL, i.e. AML-M3, AML-M8 and MPAL requiring individual therapy approach. mirna.sucic@yahoo.com 65 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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