4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 82 Klinička citologija - Usmena predavanja PNEUMOCYSTIS CARINII IN THE TRANSBRONCHIAL LUNG BIOPSY IMPRINT OF THE PATIENT NOT KNOWN TO BE HIV INFECTED- CASE REPORT Juroš Z1 , Smojver Ježek S1 , Vrabec Branica B1 , Alerić I1 and Križanac Š2 1 Department of Cytology and Department of Pneumology, University Hospital for Lung Diseases Jordanovac, Zagreb, Croatia 2 Department of Clinical Pathology, Dubrava University Hospital, Zagreb, Croatia Patients with HIV infection are exposed to the different opportunistic infections and the most commom among them is Pneumocystis. Over 85% of HIV patients without treatment would eventually develope Pneumocystis pneumonia which still causes death in about 10% of cases. Diagnosis may be suspected by the patient’s clinical presentation in typical cases but the best diagnostic yield is achieved by analyzing samples obtained by bronchoalveolar lavage and induced sputum. We present the case of 42 years-old male with long-time period undiagnosed and untreated Pneumocystis infection.First morphological diagnosis was established in transbronchial lung biopsy imprint cytology. zrinka.juros@zg.t-com.hr
Clinical Cytology - Oral Presentations MULTIMODAL IMAGE ANALYSIS OF CHRONIC LEUKEMIC LYMPHOPROLIFERATIVE DISORDERS AND THE HYPOTHESIS OF „SINGLE“ AND „MULTIPLE“ PROGRAMMED STOPS IN THE DEVELOPMENT OF TYPICAL AND ATYPICAL FORMS OF LEUKAEMIAS AND LYMPHOMAS Kardum-Skelin I1 , Radić-Krišto D1 , Jelić Puškarić B1 , Jakšić O2 , Kardum M3 , Jakšić B1 1 University Hospital Merkur, Zagreb 2 University Hospital Dubrava, Zagreb 3 Faculty of Veterinary Medicine The analysis consisted of morphometric studies, assessment of the nucleolar organization region (AgNOR) characteristics, and image cytometry (ICM), performed in 71895 cells on a SFORM PC (VAMSTEC, Zagreb). Correlation between morphometric, AgNOR and ICM characteristics revealed the low proliferative cells to possess small, homogeneous AgNOR, with the majority of cells in the peak of DNA histogram. The high proliferative cells had inhomogeneous AgNOR, mostly containing greater DNA amount than peak cells, or pathologic mitoses (DNA >4N), or the majority of cells being in the Sphase of the cell cycle. Cells with medium proliferative activity and annular AgNOR were in-between. Analysis of different tumor mass compartments showed the lymphatic cells with affinity to accumulation in bone marrow to regularly exhibit low proliferative activity (a lower percentage of cells in SFC and highest percentage of cells in the peak of the G0/ G1 phase). The cells in lymph nodes, exhibiting the characteristics of proliferative cells (an increased number of AgNOR, larger more proliferative inhomogeous AgNOR and lowest percentage of cells in the G0/G1 phase). The migration of cells from bone marrow to lymph nodes and between lymph nodes occurs in peripheral blood (a mixture of cells with low and high proliferative activity: a higher proportion of cells in SFC and at the same time in the G0/G1 phase of the cell cycle). Analysis of cell size and proliferative activity in different compartments of tumor mass revealed the cells in bone marrow and peripheral blood did not differ substantially according to size and proliferative activity, an inverse pattern was observed between peripheral blood and lymph node. As small cells are inactive and larger cells more proliferative, the analysis quite unexpectedly showed the peripheral blood cells to be largest and most inactive, in contrast to lymph node where the cells were smallest and most active. The “single” and “multiple programmed stops” in the development of typical forms of leukemias and lymphomas, subacute and and subchronic leukemias were hypothesized. ikardum@hi.t-com.hr 83 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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