4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 142 Klinička citologija - Posteri INTRAOPERATIVE IMPRINT CYTOLOGICAL ASSESSMENT OF THE SUBAREOLAR TISSUE OF THE NIPPLE AREOLA COMPLEX (NAC) Tomasović-Lončarić Č1 ,Milanović R2 , Lambaša S1 , Križanac Š1 , Štoos-Veić T3 ,Kaić G3 , Trutin Ostović K3 1 University Hospital Dubrava, Department of Pathology, Zagreb, Croatia 2University Hospital Dubrava, Department of Plastic, Reconstructive and Aesthetic Surgery, Zagreb, Croatia 3University Hospital Dubrava, Department of Clinical Cytology and Cytometry, Zagreb, Croatia One of the criteria of selection for skin sparing mastectomy (SSM) with nipple areola complex (NAC) preservation is to exclude the neoplastic involvement of subareolar tissue (NAC base) in order to minimize the possibility of local recurrence. The most common way to assess the possible neoplastic involvement is intraoperative frozen section of the NAC base tissue. Because of its limitations, particularly the false negative results due to unsampling, we tried to use intraoperative imprint cytology for more thorough intraoperative assessment. The aim was to evaluate diagnostic accuracy of this method and possibility to substitute frozen section for intraoperative assessment of NAC base A prospective clinical study was conducted of 208 consecutive female patients who underwent open biopsy because of carcinoma. Intraoperative imprints were taken from the excised subareolar tissue which was then routinely processed for definitive histology. Intraoperative imprint findings were compared with the definitive histology of the NAC base. Our results with 7.5% false negative rate, 9.8% false positive rate, sensitivity of 50% and specificity of 87.58% argue that imprint cytology might not be sufficient as an exclusive method for the intraoperative assessment of the NAC base though it should be used routinely in conjuction with frozen section examination. ctomasov@kbd.hr
Clinical Cytology - Posters ISOLATION AND CHARACTERIZATION OF LIVER CANCER STEM CELLS FROM A HEPATOCELLULAR CARCINOMA BIOPSY Tomuleasa C1 , Soritãu O1 , Páll E2 , Susman S3 , Rus D4 , Mihu C4 , Iancu C5 1Ion Chiricuta Oncology Institute, Department of Cancer Immunology, Cluj Napoca, Romania 2 University of Veterinary Medicine, Department of Obstretics and Gynecology, Cluj Napoca, Romania 3Iuliu Hatieganu University of Medicine and Pharmacy, Department of Histology, Cluj Napoca, Romania 4Iuliu Hatieganu University of Medicine and Pharmacy, Department of Pathology, Cluj Napoca, Romania 5Iuliu Hatieganu University of Medicine and Pharmacy, Department of Abdominal Sugery, Cluj Napoca, Romania Cancers are composed of heterogeneous cell populations, including highly proliferative immature precursors and differentiated cells. Recently, eloquent studies have provided proofs that cancers originate from cancer stem cells, their discovery in both solid and non-solid tumors changing our view of carcinogenesis and chemotherapy. Cancer stem cells exist in a wide array of tumours and are becoming increasingly important to understand the molecular mechanisms that regulate self-renewal and differentiation. We have isolated hepatocellular cancer stem cells from a liver biopsy, in an effort to unreveal more details about liver carcinogenesis, very important for the early detection and understanding of the mechanisms responsable for the highly aggressive clinical picture of hepatocellular carcinoma. In the current study, liver cancer stem cells were isolated from a surgical specimen of hepatocellular carcinoma in a patient whose malignancy had progresses during the previous six months. At passage 3, cells had a spindle-shaped morphology, formed spheroids and were resitant to chemotherapy agents. Immunocytochemistry staining and Reverse Transcriptase - Polymerase Chain Reaction showed that cells were pozitive for stem cell specific markers (CD 90, Oct 3/4, Nanog, SOX2, CD 133, CK19). Cells were also injected subcutaneously into CD1 mice to observe tumor formation. The cells faithfully retained the characteristics of their original tumors and provide a reliable resource for investigating the mechanisms of formation and recurrence of human liver cancer. Such investigatins may eventually have major impacts on the understanding and treatment of human cancer and metastasis. ciprian_tomuleasa@yahoo.com 143 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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