4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

Recommendations

Info

4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije ANALYSIS OF IKAROS FAMILY SPLICING VARIANTS IN HUMAN HEMATOPOIETIC LINEAGES Matulić M1 , Paradžik M2 , Jelić Puškarić B3 , Stipić J4 , Antica M2 1 Faculty of Natural Sciences, Zagreb, Croatia 1 Rudjer Boskovic Institute, Zagreb, Croatia 3 Merkur University Hospital, Zagreb, Croatia 4 Clinical Hospital Center Zagreb, Croatia Introduction: Transcription factors from the Ikaros family are involved in lymphocyte differentiation and have a critical role at specific check points of the haemopoietic pathway. However, how developmentally regulated changes are reflected in gene expression programs of lymphocyte differentiation is not well understood. It has been suggested that disregulation of transcription factors from the Ikaros family is associated with the development of different human leukemias. Aims: In this work we analyzed the state of Ikaros family members in different leukemic cells with the aim to explore the transcriptional control of human hematopoietic lineages and shed some new light on our understanding of transcription factor significance in human leukemias. Methods: By means of RT-PCR and specific primers we investigated the expression of Ikaros, Aiolos and Helios transcription factors and their splicing variants in seven leukemia cell lines derived from different types of leukemia (ALL, CML, AML) and lymphoma (histiocytic lymphoma, Burkitt lymphoma and anaplastic large cell lymphoma). Results: In all of the cell lines examined Ikaros was present in dominant Ik1 to Ik4 isoforms and small Ik6 isoform was absent. Aiolos was expressed in the majority of the cell lines, of both, B and T origin, in the form of the full length Aio1. Helios was also present only in two long isoforms Hel1 and Hel2, and was absent in one third of the lines. Conclusion: Similar distribution of positive and negative expression of Aiolos and Helios found in various types of leukemias could implicate common pathways of their regulation. antica@irb.hr 166 Analitička citologija - Posteri
Analytical Cytology - Posters MONOCYTES IN METABOLIC DISORDERS - OPPORTUNITIES FOR FLOW CYTOMETRY CONTRIBUTION Pandžić Jakšić V1 , Gizdić B2 , Miletić Z2 , Trutin Ostović K2 , Jakšić O3 1 Department of Endocrinology and Metabolism, Dubrava University Hospital, Zagreb, Croatia 2 Department of Clinical Cytology and Cytometry, Dubrava University Hospital, Zagreb, Croatia 3 Department of Hematology, Dubrava University Hospital, Zagreb, Croatia Chronic inflammation has arised as a major underlying cause of atherosclerosis, obesity and diabetes. It is mediated by cells of innate immune system like macrophages but also by their antecedents, monocytes in circulation. Roles of monocyte subsets and different markers of monocyte activation in the context of metabolic disorders have been reviewed. Applying cell based approach through flow cytometry in this field has resulted with new understanding of pathophysiologic mechanisms. Possible implications of these insights in diagnosis, prognosis and revealing of therapeutic targets in metabolic disorders remain a challenge for future. vpandzic@kbd.hr 167 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
Page 1:
4. Hrvatski kongres kliniËke citol
Page 4 and 5:
Page 6 and 7:
Page 8 and 9:
Page 10 and 11:
Page 13 and 14:
Final programme and abstract book
Page 15:
UTORAK, 13.10.2009. SRIJEDA, 14.10.
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119: 4. Hrvatski kongres kliniËke citol
Page 194: 4. Hrvatski kongres kliniËke citol
show all

4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?