4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 88 Klinička citologija - Usmena predavanja DIGITAL MORPHOMETRY OF CYTOLOGIC ASPIRATE ENDOMETRIAL SAMPLES Mahovlić V1 , Ovanin-Rakić A1 , Škopljanac-Mačina L1 , Barišić A1 , Rajhvajn S1 , Jurič D1 , Šamija I1 , Ilić-Forko J2 , Babić D2 , Škrablin-Kučić S3 , Božikov J4 1Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, Zagreb University Hospital Center, Zagreb, Croatia 2Department of Gynecologic and Perinatal Pathology, University Department of Clinical Pathology, Zagreb University Hospital Center, Zagreb, Croatia 3Department of Perinatal Medicine, University Department of Gynecology and Obstetrics, Zagreb University Hospital Center, Zagreb, Croatia 4Andrija Štampar School of Public Health, School of Medicine, University of Zagreb, Zagreb, Croatia Unlike cervical cytology, morphological cytology criteria in the differential diagnosis of endometrium have not yet been clearly defined, and methods to allow for more precise evaluation of endometrium status have been searched for. Aim. To assess the value of morphometric nucleus analysis of cytologic aspirate endometrial samples in proliferative, hyperplastic and malignant endometrium by use of digital image analysis. Materials and methods. Morphometric analysis was performed on archival cytologic aspirate endometrial samples (at least 10 per group) stained according to Papanicolaou (n=77) and May-Grünwald-Giemsa (MGG; n=80) with the following histopathologic diagnoses: proliferative endometrium, hyperplasia simplex, hyperplasia complex, hyperplasia complex atypica, and adenocarcinoma endometriodes endometrii (grade I, II and III). Interactive image analysis (nuclear area, convex area, perimeter, maximum and minimum radius, length and breadth, as well as nucleus form factor and elongation factor) was performed by use of the SFORM software (VAMSTEC, Zagreb) on at least 50 (Papanicolaou stain) and 100 (MGG stain) well preserved endometrial epithelial cell nuclei without overlapping, at magnification of X1000. Statistical data analysis was done by use of the Statistica Ver. 6 statistical package. Results. Multivariate analysis (ANOVA) distinguished malignant, hyperplastic and proliferative endometrium according to all morphometric variables with both staining methods (P0.05) from atypical hyperplasia, adenocarcinoma and proliferative endometrium only according to the nucleus form factor and elongation factor (Papanicolaou stain), whereas malignant and atypical hyperplastic endometrium (MGG stain) differed statistically significantly (P0.05). According to the cytologic staining method, morphometric parameters were considerably higher in MGG stained endometrial samples, reaching the level of statistical significance (P0.05) in the groups of hyperplasia simplex and complex, well differentiated adenocarcinoma (form factor) and atypical hyperplasia (elongation factor). Conclusion. A combination of cytomorphology and the morphometric variables assessed in this study can yield useful information on the cytologic state of endometrium, with special reference to the possible differentiation of the group of hyperplasia without atypia from the group of adenocarcinoma and atypical hyperplasia. vesna.mahovlic@zg.t-com.hr
Clinical Cytology - Oral Presentations T-LYMPHOBLASTIC LYMPHOMA WITH AN UNUSUAL t(8;14)(q24;q11) - CASE REPORT Mandac I1 , Ostojić-Kolonić S1 , Vrhovac R1 , Lasan-Trčić R2 , Jakelić-Piteša J3 , Kardum-Skelin I1 1 University Hospital Merkur, Zagreb, Croatia 2 Clinical Hospital Zagreb, Zagreb, Croatia 3 Clinical Hospital Split, Split, Croatia The cytogenetic abnormalities seen at presentation of acute lymphoblastic leukemia or lymphoblastic lymphoma (ALL/LBL) are associated with distinct clinical and hematologic disease entities. T-ALL/LBL are morphologically indinguishable from those of B-ALL/LBL. An abnormal kariotype is found in 50-70% of cases of T-ALL/LBL. Here we present a 35-year old male patient with T-ALL/LBL and t(8;14)(q24;q11.2). Our patient presented with B-symptoms, bulky mediastinal disease and CNS infiltration. Bone marrow was morphologically normal without clonal aberations in bone marrow cytogenetics. Cytological findings of the supraclavicular lymph node showed numerous CD3 positive (100%) and CD2 positive (88%) lymphoblasts which were negative for CD34 and CD10. Flow cytometry of lymph node showed T cell phenotype of immature cells: CD45 +CD2+CD5+CD7+CD4+CD8+CD3cyt+CD3TdT+CD10-CD34-HLAD/DR-. Cytogenetic analysis of lymph node showed translocation t(1;4)(p32;p12), t(8;14)(q24;q11.2). Southern blot analysis of extracted DNA from the supraclavicular lymph node demonstrated clonal rearrangement of the T cell antigen receptor (TCR/J) gene (region Vb+Jb2). Based on these findings, diagnosis of T lymphoblastic non Hodgkin lymphoma was established. Lumbal puncture and liquor analysis showed CNS infiltration with 49% lymphoblasts positive for CD4 and CD8. The disease progressed rapidly and response to therapy was poor. T-ALL/LBL with an unusual t(8;14)(q24;q11.2) is a very rare hematologic disorder with rapid disease progression and poor response to conventional therapy because of frequent central nervous system involvement and early relapses. imandac@yahoo.com 89 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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