4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 140 Klinička citologija - Posteri CHRONIC LYMPHOCYTIC LEUKAEMIA (CLL) - TRANSFORMATION TO HODGKIN LYMPHOMA OR COMPOSITE TUMOR - CASE REPORT Škrtić A1 , Borovečki A1 , Milas M2 , Džebro S1 1 University Hospital „Merkur“, Department of Pathology and Cytology, Zagreb, Croatia 2University Hospital „Merkur“, Department of Medicine, Laboratory of Cytology and Haemathology, Zagreb, Croatia Over time, 2-8% of patients with CLL develop DLBCL and
Clinical Cytology - Posters FINE NEEDLE ASPIRATION OF INTRA-ABDOMINAL LYMPH NODES IN THE DIAGNOSIS AND FOLLOW UP OF MALIGNANT LYMPHOMA Šušterčić D1 , Kardum-Skelin I1 , Jelić-Puškarić B1 , Milas M1 , Odak D2 , Vidjak V2 , Minigo H3 , Planinc-Peraica A3 , Radić-Krišto D3 , Ostojić Kolonić S3 , Jakšić B3 1Merkur University Hospital, Department of Medicine, Laboratory for Cytology and Hematology, Zagreb, Croatia 2 Merkur University Hospital, Department of Diagnostic and Interventional Radiology 3 Merkur University Hospital, Department of Medicine, Zagreb, Croatia Aim of the study. To assess appropriateness of the material obtained by fine-needle biopsy of intra-abdominal lymph nodes and the possibility of malignant lymphoma differentiation from other malignant tumors by cytomorphology supplemented by smear immunocytochemistry and flow cytometry using cytogenetic and molecular analysis (PCR) of cell suspension of sample obtained by fine-needle biopsy. Patients and methods. During a 10-year period, 242 intra-abdominal lymph nodes were examined. US-guided biopsy was done in 226 and CT-guided biopsy in two cases; biopsy was done blindly in only one patient. Biopsy was performed by 22-gauge CHIBA needle, length 15-20 cm, depending on lesion localization. Samples were analyzed on May-Grünwald-Giemsa stained smears by determination of cellular markers by smear immunocytochemistry and flow cytometry using cytochemical, cytogenetic and/or molecular analysis (PCR). Results. The material was inadequate for cytologic analysis in 8 cases. Malignant lymphoma was found in 97 patients (non-Hodgkin’s lymphoma (NHL) in 85 and Hodgkin’s lymphoma (HL) in 12 cases). In 81 patients, the diagnosis of malignant lymphoma was made for the first time (NHL in 72 and HL in nine patients). In cases of newly detected lymphoma, diagnostic work-up was supplemented by smear immunocytochemistry in 66, phenotyping on flow cytometer in 48, PCR in 13 and FISH in two cases. Out of 16 patients with previously diagnosed malignant lymphoma, NHL was present in 13 and HL in three cases. A tumor of another genesis was detected in three patients with previously diagnosed lymphoma (carcinoma in two patients with HL and bronchial small cell carcinoma in one patient with NHL), acute inflammation was found in 14 and elements of granulomatous lesion in three patients. Conclusion. Visualization of intra-abdominal lymph nodes by imaging methods enables target sampling for cytologic analysis as a reliable method in the diagnosis and follow up of malignant lymphoma, when supplemented by some adjuvant sophisticated technologies. dunjasustercic@yahoo.com 141 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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