4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije
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Klinička citologija - Posteri
DEXAMETHASONE AND CHITOSAN REDUCE HEPATIC FIBROSIS AND INFLAMATIO
IN WISTAR RATS WITH BILE DUCT LIGATION
Trip DM1 , Olteanu D1 , Filip A1 , Clichici S1 , Muresan A1 , Sofronie A1 , Nagy A2 ,
Moldovan R2 1Iuliu Hatieganu University of Medicine and Pharmacy, Department of Physiology,
Cluj Napoca, Romania
2University of Veterinary Medicine and Sciences, Department of Pathology, Cluj Napoca,
Romania
Introduction:Chronic liver disease is characterized by excessive deposition of extracellular
matrix in the liver during chronic injury. During early stages of this disease, cells
begin to synthesize and secrete profibrotic proteins that stimulate matrix production
and inhibit matrix degradation. Although it is clear that this process is important for the
development of fibrosis, what remains unknown are the effects of dexamethasone and
chitosan on cholestasis and subsequent fibrosis, induced by common bile duct ligation.
Material and Methods:A total of 30 female Wistar rats were used in this study. The control
group underwent laparotomy and the bile duct was dissected. Experimental groups
received various daily doses of dexamethasone intramuscular and chitosan orogastric
for 6 days after biliary duct ligation. The liver and serum was analyzed for reactive oxygen
species levels and the serum for usual liver function tests after 6 days of treatment.
Also histopathological examination was performed using a histological scoring system.
Results:The levels of MDA in the liver were significantly lower in the groups treated with
dexamethasone compare to the group that underwent only biliary duct ligation (p=0,008)
the same was seen in the group with chitosan (p=0,027) and chitosan with dexamethasone
(p=0,014). Biliary duct ligation induced severe cholestasis, confirmed by altered
liver function, portal and focal inflammation, necrosis and fibrosis.
Conclusion: Dexamethasone and chitosan reversed all the biochemical parameters used
to quantify liver pathophysiology and ameliorated histopathological changes previously
induced by BDL. Findings of the present study suggest that this treatment may favor
collagenolytic activity, an assumption supported by amelioration of necroinflammatory
liver injury and fibrogenesis.
dora.trip@yahoo.com