4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta
4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta
4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta
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Clinical Cytology - Posters<br />
ISOLATION AND CHARACTERIZATION OF LIVER CANCER STEM CELLS FROM A<br />
HEPATOCELLULAR CARCINOMA BIOPSY<br />
Tomuleasa C1 , Soritãu O1 , Páll E2 , Susman S3 , Rus D4 , Mihu C4 , Iancu C5 1Ion Chiricuta Oncology Institute, Department of Cancer Immunology, Cluj Napoca,<br />
Romania<br />
2 University of Veterinary Medicine, Department of Obstretics and Gynecology,<br />
Cluj Napoca, Romania<br />
3Iuliu Hatieganu University of Medicine and Pharmacy, Department of Histology,<br />
Cluj Napoca, Romania<br />
4Iuliu Hatieganu University of Medicine and Pharmacy, Department of Pathology,<br />
Cluj Napoca, Romania<br />
5Iuliu Hatieganu University of Medicine and Pharmacy, Department of Abdominal<br />
Sugery, Cluj Napoca, Romania<br />
Cancers are composed of heterogeneous cell populations, including highly proliferative<br />
immature precursors and differentiated cells. Recently, eloquent studies have provided<br />
proofs that cancers originate from cancer stem cells, their discovery in both solid and<br />
non-solid tumors changing our view of carcinogenesis and chemotherapy. Cancer stem<br />
cells exist in a wide array of tumours and are becoming increasingly important to understand<br />
the molecular mechanisms that regulate self-renewal and differentiation. We<br />
have isolated hepatocellular cancer stem cells from a liver biopsy, in an effort to unreveal<br />
more details about liver carcinogenesis, very important for the early detection and<br />
understanding of the mechanisms responsable for the highly aggressive clinical picture<br />
of hepatocellular carcinoma.<br />
In the current study, liver cancer stem cells were isolated from a surgical specimen<br />
of hepatocellular carcinoma in a patient whose malignancy had progresses during the<br />
previous six months. At passage 3, cells had a spindle-shaped morphology, formed<br />
spheroids and were resitant to chemotherapy agents. Immunocytochemistry staining<br />
and Reverse Transcriptase - Polymerase Chain Reaction showed that cells were pozitive<br />
for stem cell specific markers (CD 90, Oct 3/4, Nanog, SOX2, CD 133, CK19). Cells were<br />
also injected subcutaneously into CD1 mice to observe tumor formation.<br />
The cells faithfully retained the characteristics of their original tumors and provide a reliable<br />
resource for investigating the mechanisms of formation and recurrence of human<br />
liver cancer. Such investigatins may eventually have major impacts on the understanding<br />
and treatment of human cancer and metastasis.<br />
ciprian_tomuleasa@yahoo.com<br />
143<br />
4 th <strong>Croatian</strong> <strong>Congress</strong> of Clinical Cytology / 1 st <strong>Croatian</strong> Symposium of Analytical Cytology / 2 nd <strong>Croatian</strong> Symposium of Cytotechnology