4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 112 Klinička citologija - Usmena predavanja THE IMPORTANCE OF URGENT CYTOLOGICAL EXAMINATION OF SYNOVIAL FLUIDS IN DIFFERENTIATION INFLAMMATORY AND NON-INFAMMATORY DISEASES Trutin Ostović K1 , Kaić G1 , Ostović I2 , Škoro M1 , Novak N-P1 , Morović-Vergles J3 1Dubrava University Hospital, Department of Clinical Cytology and Cytometry, Zagreb, Croatia 2Faculty of Humanities and Social Sciences, University of Zagreb, Department of Psychology, Zagreb, Croatia 3Dubrava University Hospital, Department of Clinical Immunology and Rheumatology, Zagreb, Croatia Aim. To imply the possibilities of the urgent cytological examination of synovial fluids in differentiation of arthropathies and to motivate clinicians to use this method. Methods. This examination consists of macroscopic analysis (volume, colour, clarity, viscosity and mucin clot test), native microscopic analysis for crystals, counting total nucleated cell count in Bürker-Türk counting chamber and microscopic analysis rapid Hemacolor stained smears for neutrophil granulocyte percentage. All these analyses are done within one hour since FNA. Results. The study includes 115 synovial fluids (81 with inflammation, 34 with non-inflammation) obtained by FNA between 2003 and 2008. The clarity, viscosity, mucin clot test, the total nucleated cell count and the neutrophil granulocyte percentage enables distinction between inflammation and non-inflammation with statistically significant difference at the 0.01 level. Crystals (monosodium urate) are detected in 12 synovial fluids and we can diagnose gout. Conclusion. The urgent cytological analysis of the synovial fluid is useful basic diagnostic screening test in differentiation inflammatory and non-inflammatory joint diseases and we recommend using it as the initial test in the diagnostic procedure of these illnesses using our protocol. ktrutin@kbd.hr
Clinical Cytology - Oral Presentations CERVICAL CYTOLOGY AND HPV TEST IN FOLLOW-UP AFTER CONISATION OR LLETZ Verša Ostojić D1 , Vrdoljak-Mozetič D1 , Štemberger-Papić S1 , Finderle A2 , Eminović S3 1 Department of Gynecological Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Rijeka, Croatia 2 Department of Perinatology, University Department of Gynecology and Obstetrics, University Hospital Center Rijeka, Croatia 3 Department of Pathology, School of Medicine, University of Rijeka, Croatia Objective: To determine cytology-histology correlation after conisation or Large Loop Excision of the Transformation Zone (LLETZ) for cervical intraepithelial neoplasia (CIN), resection margin status, compliance to the follow-up protocol and evaluation of cervical cytology and HPV testing in two years period after surgical treatment. Materials and Methods: We made a retrospective analysis of the case records of 251 conisations and LLETZ performed between January and December 2006. The conventional cervical smears were analysed and digene Hybrid capture 2 test was used for detection of 13 high-risk HPV types. Results: Histology analysis demonstrated CIN1+ lesion in 234 cases (93,2%) with cytology-histology correlation in 97,9 % of cases. Preoperative HPV test was made in 142 histology confirmed CIN1+ lesions and 137 (96,5%) tested positive. The resection margins were involved in 48 (35,8%) cases and in 24 (10,2%) the margins were difficult to determine. 217 (86,5%) patients attended the posttreatment visits and abnormal cytology was found in 33 (15,2%) cases. The post-treatment HPV test was performed in 159 women and was positive in 25 (15,7%) cases. The complete follow-up control cytology, with at least three Pap smears in two years or with second treatment was found in only 146 (58,2 %) patients. 14/217 (6,5 %) patients underwent second treatment with histology confirmed treatment failure. In all patients where control smear was performed, repeated cytology found HSIL. In six women control HPV test was performed, in five cases it was positive and in one case with VAIN2 on biopsy of vagina it was negative. Conclusion: Our study confirme important place of cervical cytology in the diagnisis of cervical intraepitelial lesions and monitoring after treatment. In the future we have to improve compliance to the follow-up protocols and use of HPV test in the selection of women at risk of treatment failure. dversa.ostojic@gmail.com 113 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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