4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 110 Klinička citologija - Usmena predavanja IDENTIFICATION OF BRAIN CANCER STEM CELLS IN WHO STAGE IV BRAIN TUMOURS Tomuleasa C1 Soritãu O1 , Foris V1 , Ioani H3 , Ciucã DR2 , Susman S2 , Mihu C2 , Florian IS3 1Ion Chiricuta Oncology Institute, Department of Cancer Immunology, Cluj Napoca, Romania 2Department of Pathology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania 3Department of Neurosurgery, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj, Npoca, Romania Tumours of the nervous system represent the leading cause of cancer-related death in children and the fourth leading cause in adults. Despite decades of research and clinical experience, the life expectancy of an adult diagnosed with a high-grade brain tumour is typically less than one year. Our ability to develop new therapies for these deadly cancers is highly dependent on an improved understanding of the molecular and cellular changes that contribute to their development and continued growth. Brain cancers comprise a complex microcosm of both neoplastic and non-neoplastic cells that each have distinct roles in dictating tumor formation and growth. The most important of these cells are cancer stem cells, responsable for post-radiochemotherapy relapse and progression. We cultured 5 fresh surgical specimens of human glioblastoma multiforme using the same protocols used for culturing bone marrow mesenchymal stem cells. At passage 3, cells had a spindle-shaped morphology, formed spheroids and were resitant to chemotherapy agents. Immunocytochemistry staining and Reverse Transcriptase – Polymerase Chain Reaction showed that cells expressed CD 133 cancer stem cell specific marker. The identification of brain tumour stem cells provides a powerful tool to investigate the tumorigenic process in the central nervous system and to develop targeted therapies, capable of increasing the survival of patients diagnosed with WHO grade four brain cancers. ciprian_tomuleasa@yahoo.com
Clinical Cytology - Oral Presentations DEDIFFERENTIATION THERAPY AND DARWINISM FOR SORTING OF OVARIAN CANCER STEM CELLS Tomuleasa C1 , Soritãu O1 , Ciucã DR2 , Susman S2 , Mihu C2 1Ion Chiricuta Oncology Institute, Department of Cancer Immunology, Cluj Napoca, Romania 2Iuliu Hatieganu University of Medicine and Pharmacy, Department of Pathology, Cluj Napoca, Romania Introduction. The cellular mechanisms underlying the increasing aggressiveness associated with ovarian cancer progression are poorly understood. Coupled with a lack of identification of specific markers that could aid early diagnoses, the disease becomes a major cause of cancer-related mortality in women. The high incidence of recurrence attributable to multidrug resistance and the multiple histologic phenotypes indicative of multipotency suggest a stem-like etiology of ovarian cancer. Here we adopted suspension culture combined with anti-cancer regimens as a strategy for screening ovarian cancer stem cells. These tumor stem cells could survive and be highly enriched in non-adherent suspension culture while chemotherapeutic agents could destroy most rapidly dividing cancer cells and spare relatively quiescent cancer stem cells. Material and Methods. MLS human ovarian cancer cells were cultured in serum-free medium, suplemented with Epidermal Growth Factor, basic Fibroblast Growth Factor, B27 and N2. Cells of passage 8 were treated in combination with anticancer agents Doxorubicin and Carboplatin at different peak plasma concentrations for 24 hours, and then maintained under suspension culture. The stem-like properties were confirmed by immunocytochemistry staining techniques, by multidrug resistance assays and by their ability to grow in an anchorage-independent manner in vitro as spheroids. Selected cells were also injected subcutaneously into CD1 mice to observe tumour formation. Results and Discussion.Here we present direct evidence that the aggressiveness of human ovarian cancer may be a result of transformation and dysfunction of stem cells in the ovary. The tumorigenic clones, even on serial transplantation continue to establish tumours, theraby confirming their identity as tumor stem cells. Suspension culture combined with anticancer regimens provides an effective means of isolating, culturing and purifying ovarian cancer stem cells. ciprian_tomuleasa@yahoo.com 111 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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