4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije PRESENTATION OF INTEGRATED MODULE FOR INPUT AND PROCESSING OF CYTO- LOGICAL REPORTS WITHIN THE COMPUTER PROGRAMME “RIBIS“ Markanjević T1 , Vunić S2 1 University Hospital Center Rijeka, Croatia 2 ‘SING’ company for computer engineering, Rijeka, Croatia Objective: The presentation of computer programme for input, processing and assesing of cytological findings stressing the Pap test results. Methods: In collaboration of Department of Gynecological Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Rijeka, Croatia and ‘SING’ company for computer engineering from Rijeka we made and improve a computer programme for input and processing of cytological findings. The input of Pap tests is based on the classification ‘ZAGREB 2002’. The programme is adapted to the workprocess of cytological laboratory from the reception of referral slides to the issuing of reports in print. By means of this programme data base of all the patients is created. This ensures a simple insight into the previous Pap test findings and patients’ chart. It also ensures the input of other cytological findings as well as HPV tests performed on our department. The programme has an option of statistical data processing - the performance of cytotechnologist and specialists in clinical cytology, a number of daily examined slides, a share of unsarisfactoty samples and a number of negative and abnormal findings with the possibility of subcategorisation. The Department of Gynaecological cytology operates interactively with the Department of Gynaecological Polyclinic and other departments in the Clinic for Obstretics and Gynaecology thus the request forms are sent electronically. We also have an insight into the electronic patient charts containing the information about medical history as well as all the diagnostic procedures and findings. We also operate interactivelly with the Department of Pathology and we have the access to pathology results of gynaecological patients. Results: By means of an example of normal and abnormal Pap test we will demonstrate the sequence of procedures using computer programme: the input of general and clinical data, the part performed by cytotechnologiests along with rescreening, the part performed by doctors, print-out of the results and the patient chart. Conclusion: Computerization of the Department of Gynaecological cytology contributed to the effectiveness of daily work and the quality of processing and evaluation od cytological findings with the possibility of improved quality control. tina.valencic@ri.t-com.hr 176 Citotehnologija - Usmena predavanja
Cytotechnology - Oral Presentations PROBLEMS WITH STAINING PROCEDURES IN IMMUNOCYTOCHEMISTRY Meandžija R1 , Fumić G1 , Naglić G1 , Štanfel O2 , Seili-Bekafigo I1 1Department of Cytology, Department of Internal Medicine, Clinical Hospital Center Rijeka, Croatia 2 Rijeka University School of Medicine, Department of Pathology, Rijeka, Croatia Aim: To identify weaknesses of immunocytochemistry (ICK) staining procedures, and to propose solutions for some of them, since data in the literature are very scarce on that subject. Material and methods: During 3 years period in The Department for Cytology, Department of Internal Medicine, Clinical Hospital Center Rijeka, we performed totally 475 ICK analyses for 151 patients, mean 3,15 per patient. Most monoclonal antibodies used (Ber-ep4, CD3, CD20, Calretinin) react with membrane-bound antigenes, so any kind of pretreatment was not applied. We also tried to show TTF positivity in primary lung tumors, with and without pretreatment with TRIS- EDTA puffer on 96-97oC. Results: Staining for membrane-bound antigenes, gave good results using standard protocols recommended by manufacturer. The best results were obtained when using special Polysine slides. After pretreatment we got positive reaction with the nuclear antigen TTF as well. Conclusion: ICK is a powerfull diagnostic tool, but the staining procedure itself has to be precisely defined and standardized. The most important problems are: quality of particular slides (we do not know in advance if there are enough well preserved cells on each given slide), maintaining of the specimen on the slide during staining procedure if standard slides are used, correct determination of dillution and incubation period for each monoclonal antibody, revealing of the nuclear antigenes without interfering with specimen quality, and lack of control slides. There is a lot of work to be done in this field, regarding standardization and quality control. rmeandzija@net.hr 177 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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