4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 124 Klinička citologija - Posteri RHABDOMYOSARCOMA WITH BONE MARROW INFILTRATION MIMICKING HEMATOLOGIC NEOPLASIA. CASE REPORT Jelić-Puškarić B1 , Rajković-Molek K2 , Raić Lj3 , Batinić D4 , Konja J3 , Kardum-Skelin I1 1 Merkur University Hospital, Department of Medicine, Zagreb; 2 University Hospital Center Rijeka, Department of Medicine, Rijeka; 3 University Hospital Center Zagreb, Department of Pediatrics, Zagreb; 4 University Hospital Center Zagreb, Department of Laboratory Diagnosis, Zagreb, Croatia Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children younger than 15 years. According to the World Health Organization, there are embryonal, alveolar and pleomorphic types of RMS. Most RMS patients present with a tumor mass in the head and neck region, urogenital tract or lower extremities. Unusual clinical presentation of the disease with massive bone marrow infiltration at the disease onset and mimicking hematologic neoplasm is rarely seen. A case is presented of a 14-yearold, previously healthy girl hospitalized for outpatiently detected leukocyte elevation (L 24.8x109/L). For two weeks before, she had complained of fatigue, myalgia and frequent bruising. On admission, clinical examination revealed numerous petechiae and hematomas on the skin, enlarged lymph node inguinally on he left, and palpable spleen 2 cm. Laboratory findings showed leukocytosis (L 28.5x109/L), anemia (E 3.02x1012/L, Hb 85 g/L) and thrombocytopenia (Plt 15x109/L). Bone marrow biopsy produced a hypercellular bone marrow sample with suppression of all three hemocytopoiesis lines and bone marrow infiltration with numeorous immature tumor cells that were cytochemically and immunocytochemically positive for PAS, vimentin and desmin, and negative for LCA, CD20, CD19, CD3, CD10, NSE, Ber EP 4, OIL RED and ANAE. Considering the morphological, cytochemical and phenotypic characteristics, the cytologists believed it was a case of bone marrow infiltration with RMS cells. Abdominal computed tomography revealed a primary tumor occupying the entire retropeoritoneal space. Tumor biopsy confirmed it to be alveolar RMS subtype. In conclusion, in case of bone marrow infiltration with primitive, immature cells, RMS should be taken in consideration on differential diagnosis. Adjuvant technologies (e.g., cytochemistry, immunocytochemistry, cytogenetic analysis, flow cytometry, and molecular analysis) can be very helpful in this diagnostic work-up, and may even lead to definitive diagnosis in some cases. biljana.jelic.puskaric@zg.t-com.hr
Clinical Cytology - Posters IMPACT OF STATIN, ANGIOTENSIN CONVERTING ENZYME INHIBITOR, ANGIOTENSIN II RECEPTOR BLOCKER AND DIHDROPYRIDINE TREATMENT ON PERITONEAL DIALYSATE COMPOSITION Jurić K1 , Cavrić G2 , Naumovski-Mihalić S2 , Bartolek D3 , Nassabain K4 , Novak NP5 1University Hospital Dubrava, Department of Internal Medicine, Zagreb, Croatia 2University Hospital Merkur, Department of Internal Medicine, Zagreb, Croatia 3University Hospital Merkur, Department of Anaesthesiology, Reanimation and Intensive Care, Zagreb, Croatia 4 General Practice, Zagreb, Croatia 5University Hospital Dubrava, Department of Clinical Cytology and Cytometry, Zagreb, Croatia Peritoneal dyalisis is increasingly used in the treatment of end-stage renal failure. Macrophage domination in peritoneal dialysate when there are no signs of acute peritonitis can reflect increased macrophage count in peritoneum, commonly found in patients on peritoneal dialysis. This indicates that the peritoneum of these patients is a chronically inflamed organ. Numerous studies suggest the pleiotropic properties of statins, agniotensin converting enzyme inhibitors (ACEi), angiotensin II receptor blockers (ARB) and dihydropyridines. The objective of the study was to investigate the effect of these medicines on the cellular composition of the peritoneal dialysate, regarding their assumed antiinflammatory properties. In this retrospective study we analyzed 21 peritoneal dialysate samples in total of 12 patients. In the samples of the patients on statin therapy significantly higher percentage of monocytes were found in peritoneal dialysate (p=0.005). Likewise, a higher percentage of lymphocytes and a lower percentage of macrophages were found, although not yielding statistical significance (p = 0.143 for lymphocytes and p = 0.063 for macrophages). In the samples of patients receiving ACEi or ARB treatment significantly lower lymphocyte count was found (p = 0.001) and a lower percentage of monocytes, with a higher percentage of macrophages, but with no statistical significance. In the samples of patients on dihydropyridine therapy significantly higher percentage of lymphocytes was found (p = 0.004). At the same time a higher percentage of monocytes and a lower percentage of macrophages were observed, that was not significant (p = 0.077 for monocytes and p = 0.158 for macrophages). As a result of treatment with these medicines certain changes in the cellular composition of the peritoneal dialysate were observed but further larger scale studies would be warranted. kjuric@kbd.hr 125 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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