4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta
4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta
4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta
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Clinical Cytology - Posters<br />
APOPTOSIS OF LEUKEMIC CELLS - A CASE REPORT<br />
Vrbanus LJ1 , Sučić M1 , Marković-Glamočak M1 , Ries S1 , Gjadrov-Kuveždić K1 ,<br />
Fabijanić I1 , Antulov J1 , Labar B2 1 Zagreb University Clinical Hospital Center and School of Medicine, Clinical Department<br />
of Pathology and Cytology, Zagreb, Croatia<br />
2Zagreb University Clinical Hospital Center and School of Medicine, Department of<br />
Hematology, Zagreb, Croatia<br />
Transformation of leukemic cells is linked to delay of maturation, delay in apoptosis and<br />
altered responsiveness to growth factors. However, in some studies were found that<br />
FAS (CD95/APO1) that mediates apoptotic signal is more frequently expressed in M4/M5<br />
leukemic cells, and could be related to poor clinical outcome of acute myeloid leukemia<br />
(AML) patients. Apoptosis of bone marrow (BM) leukemic cells in our patient with<br />
AML-M5 could be also related to FAS expression on leukemic cells. The aim of the study<br />
was to compare cytomorphology and cytochemistry of BM apoptotic leukemic cells to<br />
preserved peripheral blood (PB) leukemic cells in our patient with AML-M5. Patients<br />
and methods: In the study is reported a 76 years old men with AML-M5 treated at Zagreb<br />
University Hospital Center. BM and PB of AL patient were analyzed after Pappenheim<br />
and cytochemical stainings and classified according FAB and WHO classification. Flow<br />
cytometry and cytogenetic analyses of BM cells were also done. Results: In BM were<br />
found numerous apoptotic cells with characteristic nuclear chromatin condensation<br />
and formation of pyknotic nuclei and only few preserved monoblasts and promoncytes.<br />
Analysis of PB revealed leukocytosis and 80-90% monocytic cells (46% monoblasts, 29%<br />
promonocytes and 11% monocytes). In almost all of BM apoptotic leukemic cells and<br />
PB leukemic cells esterase was strongly positive. Conclusion: Cytomorphology and cytochemistry<br />
of PB leukemic cells point to proliferation of immature monocytic cells and<br />
cytomorphology of BM to cell apoptosis. Cytochemistry of BM apoptotic cells confirmed<br />
monocytic cell lineage and AML-M5b was diagnosed in our patient.<br />
ljkuzmanovic@yahoo.com<br />
149<br />
4 th <strong>Croatian</strong> <strong>Congress</strong> of Clinical Cytology / 1 st <strong>Croatian</strong> Symposium of Analytical Cytology / 2 nd <strong>Croatian</strong> Symposium of Cytotechnology
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