4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

Recommendations

Info

4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije were similar between JAK2-mutant and wild-type leukemias and included alterations of RUNX1, WT1 and TP53. Loss of the JAK2 mutation by mitotic recombination, gene conversion or deletion was excluded in all wild-type AMLs. In some patients, mutations in TP53, CBL or TET2 were present in JAK2 wild-type leukemic blasts, but absent from the JAK2-mutant MPN, demonstrating that in some patients TET2 mutations are present in a clone distinct from that harboring a JAK2 mutation. By contrast in a chronic phase patient, different clones harboring mutations in JAK2 or MPL represented the progeny of a shared TET2-mutant ancestral clone. These data uncover further clonal heterogeneity in the MPN. rkusec@irb.hr CYTOGENETIC RESULTS IN 24 CASES OF MULTIPLE MYELOMA: SHORT REPORT Lasan Trčić R1 , Kardum Skelin I2 , Šušterčić D2 , Planinc-Peraica A2 , Ajduković R3 , Hariš V3 , Kušec R3 , Begović D1 1Cytogenetic Laboratory, Department of Pediatrics, University Hospital Zagreb, Zagreb, Croatia 2 Department of Internal Medicine, Merkur University Hospital, Zagreb, Croatia 3Clinical Institute of Laboratory Diagnostics, Dubrava University Hospital, Zagreb, Croatia Great studies of multiple myeloma (MM) strongly suggested that specific chromosomal changes are of prognostic significance in patients with MM1. We have performed cytogenetic analysis and recently fluorescent in situ hybridization (FISH) on 43 cases of MM. Clonal chromosomal changes were present in 24 (56%) cases. Hyperdiploid karyotype was found in 12 (50%) cases, hypodiploid in 8 (33%) cases, and 4 (17%) cases had a pseudodiploid karyotype. The most common numerical abnormalities were gains of whole chromosomes 15, 11, 3 and 6. Whole chromosome losses were also frequent involving chromosomes X, 13, 14, and 8. Most cases showed also structural rearrangments (n=17, 71%): del(1p), dup(1q), del(5q), del(13q), del(17p) and t(11;14)(q13;q32) (n=4, 17%). Chromosome -13/13q deletion was found in 42% (n=10) cases; complete loss of 13 was observed in 67% (n=7) cases, whereas 33% (n=3) had interstitial deletions. In the majority of the cases there was a mixture of abnormal and normal metaphases. lasan.ruzica@hotmail.com 156 Analitička citologija - Plenarna i pozvana predavanja
Analytical Cytology - Plenary and Invited Lectures FLOW CYTOMETRIC CLONALITY ANALYSIS OF ENDOSCOPIC ULTRASOUND-GUIDED FINE-NEEDLE ASPIRATION OF LYMPH NODES Miletić Z1 , Gizdić G2 , Štoos-Veić T1 , Kaić G1 , Novak NP1 , Tadić M3 , Jakšić O2 , Trutin Ostović K1 1Department of clinical cytology and cytometry, Dubrava University Hospital, Zagreb, Croatia 2Clinic of internal medicine, Department of hematology, Dubrava University Hospital, Zagreb, Croatia 3Clinic of internal medicine, Department of gastroenterology, Dubrava University Hospital, Zagreb, Croatia Immunophenotyping by flow cytometry (FC) is an essential tool in the diagnosis of non-Hodgkin’s lymphomas (NHLs) in fine-needle aspiration cytology (FNAC) of palpable lymph nodes or endoscopic ultrasound-guided (EUS) FNAC of deep-seated lymph nodes. Because at least 80% of NHLs are of B-cell type, detection of immunoglobulin (Ig) light-chain-restriction (clonality) represents the most commonly used evidence for a diagnosis of B-cell lymphoma. In our study we wanted to evaluate accuracy of FC clonality compared to morphologic diagnosis of EUS-FNA of deep-seated lymph nodes. Direct smears were made and selected slide was stained for rapid-on site evaluation procedure and if needed additional samples were sent for FC clonality analysis. Sixteen patients with suspicious NHL of deep-seated lymph nodes obtained by EUS-FNA were submitted for FC clonality analysis. Six of the patients had supected pancreatic lesions, 6 had abdominal and 4 had mediastinal lesion. Adequacy of samples with diagnostically relevant material were 68,7%. Monoclonality was demonstrated in 7 of 11 cases cytologically diagnosed as NHL. Polyclonality was demonstrated in 4 of 11 cases cytologically diagnosed as benign reactive lymphadenopathy. Our results show that EUS-FNAC with FC is a sensitive and specific tool in the diagnosis of deep-seated B-NHL. Successful cytologic diagnosis combined with FC clonality analysis can be performed in majority of cases and may eliminate need for open biopsy, especially in relapsed disease. zorana.miletic@kbd.hr 157 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
Page 1:
4. Hrvatski kongres kliniËke citol
Page 4 and 5:
Page 6 and 7:
Page 8 and 9:
Page 10 and 11:
Page 13 and 14:
Final programme and abstract book
Page 15:
UTORAK, 13.10.2009. SRIJEDA, 14.10.
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109: 4. Hrvatski kongres kliniËke citol
Page 194: 4. Hrvatski kongres kliniËke citol
show all

4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?