4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 146 Klinička citologija - Posteri HODGKIN’S LYMPHOMA VARIANT OF RICHTER’S SYNDROME Vasilj A1 , Kojić-Katović S1 , Maričević I1 , Žokvić E1 , Bobuš Kelčec I2 , Tomas D3,4 , Ćurić-Jurić S1 1Department of Cytology, University Clinic of Internal Medicine, Sestre Milosrdnice University Hospital, Zagreb, Croatia. 2 Department of Cytology, University Clinic for Tumors, Zagreb, Croatia. 3University Department of Pathology, Sestre Milosrdnice University Hospital, Zagreb, Croatia. 4University of Zagreb, School of Medicine, Zagreb, Croatia Chronic lymphocytic leukemia/small lymphocitic lymphoma (CLL/SLL) is low-grade malignant lymphoprolipheration, that has tendency to convert to a higher-grade neoplasam over time. More common is the development of a diffuse large cell lymphoma or transformation into prolymphocytic cell population.. In rare cases, 0,1-0,5% of patients develop multiple myeloma or Hodgkin’s lymphoma. We present 65-year old female with Hodgkin’s variant of Richter’s syndrome. On the basis of clinical simptoms, cytological, hystological and immunohistological finding in April 2008 CLL/SLL were diagnosed. The patient was treated with 8 courses of R-CHOP. After 10 month, FNA of the one of the enlarged lymph node on the neck was performed . The diagnosis was Hodgkin’s lymphoma. Immuno-hystological studies of the lymph node was consistent with type I Hodgkin’s type of Richter’s syndrome. Patient was treated with 3 courses of ABVD and radiotherapy. avasilj@kbsm.hr
Clinical Cytology - Posters THE VALUE OF URINARY DECOY CELL FINDING IN PATIENTS WITH KIDNEY TRANSPLANTATION: A SINGLE CENTER EXPERIENCE Vidas Ž1 , Mišić M2 , Pačić A4 , Jurenec F1 , Knotek M3 , Kardum-Skelin I5 1 Division of Urology, Clinical Hospital “Merkur”, Zagreb, Croatia 2 Department of Cytology, General Hospital “Dr. J. Benčević”, Slavonski Brod, Croatia 3 Department of Medicine, Clinical Hospital “Merkur”, Zagreb, Croatia 4 Department of Pathology, Clinical Hospital “Dubrava”, Zagreb, Croatia 5 Laboratory of Cytology and Hematology, Clinical Hospital “Merkur”, Zagreb, Croatia Childhood infection with polyomaviruses leads to a life-long latent infection of renal and urinary tract epithelia. Replication in the reno-urinary epithelium is associated with viral cytopathic changes such as nuclear inclusions and decoy cells (DC). Three species cause infection in humans, i.e. BK virus (BKV), JC virus (JCV), and simian virus (SV40). During the 2005-2009 period, cytological urine analysis was performed in 154 patients (94 male and 60 female) with kidney transplantation (n=19), simultaneous pancreaskidney transplantation (SPKT) (n=9) and simultaneous kidney and liver transplantation (n=2). Urine samples were analyzed according to the protocol once monthly following transplantation. Fresh urine samples were analyzed within 15 min of sampling (not the first morning void). Briefly, 0.5 mL urine was centrifuged in a cytocentrifuge at 600 rpm for 5 min and slides were stained by the methods of May-Grünwald-Giemsa (MGG) and Papanicolaou. The period from transplantation to the first occurrence of decoy cells in the urine and the period of decoy cell persistence in the urine were assessed. The presence of decoy cells (10 DC) and red blood cells (100 E) per cytospin smear was semiquantitatively determined, along with analysis of inflammatory cells (neutrophilic granulocytes) and fungi. Kidney biopsy was done according to the protocol (75% of patients), clinical indication (18% of patients), or immediately upon transplantation as so-called zero biopsy (7% of patients). In patients where decoy cells were detected, their sensitivity, specificity, and negative and positive predictive value for BK nephropathy were calculated. Correlation of the study parameters was estimated by use of Kruskal-Wallis test (Statistica 7.1). Decoy cells were found in 30 patients (20 male and 10 female), age median 40 (range 16-69) years, at a mean of day 115 (range day 5-747) of transplantation, whereas their presence was recorded for a mean of 141 (range 77- 771) days. Immunohistochemical staining of kidney biopsy sample for polyomavirus (BK type) yielded positive reaction in 2/30 (7%) patients. Erythrocyturia was present in 29/30 patients with decoy cells. Up to 20 red blood cells per cytospin smear were recorded in 14 patients, more pronounced erythrocyturia (>100 red blood cells per cytospin smear) in eight patients, and 20-100 red blood cells per cytospin smear in seven patients. The number of decoy cells per cytospin smear generally ranged less than 10 in 25/30 patients, whereas more than 10 decoy cells per cytospin smear were only recorded in 5/30 patients. There was no statistically significant correlation between the finding of neutrophilic granulocytes and fungi, and the presence of decoy cells. Immunohistochemistry 147 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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