4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 144 Klinička citologija - Posteri DEXAMETHASONE AND CHITOSAN REDUCE HEPATIC FIBROSIS AND INFLAMATIO IN WISTAR RATS WITH BILE DUCT LIGATION Trip DM1 , Olteanu D1 , Filip A1 , Clichici S1 , Muresan A1 , Sofronie A1 , Nagy A2 , Moldovan R2 1Iuliu Hatieganu University of Medicine and Pharmacy, Department of Physiology, Cluj Napoca, Romania 2University of Veterinary Medicine and Sciences, Department of Pathology, Cluj Napoca, Romania Introduction:Chronic liver disease is characterized by excessive deposition of extracellular matrix in the liver during chronic injury. During early stages of this disease, cells begin to synthesize and secrete profibrotic proteins that stimulate matrix production and inhibit matrix degradation. Although it is clear that this process is important for the development of fibrosis, what remains unknown are the effects of dexamethasone and chitosan on cholestasis and subsequent fibrosis, induced by common bile duct ligation. Material and Methods:A total of 30 female Wistar rats were used in this study. The control group underwent laparotomy and the bile duct was dissected. Experimental groups received various daily doses of dexamethasone intramuscular and chitosan orogastric for 6 days after biliary duct ligation. The liver and serum was analyzed for reactive oxygen species levels and the serum for usual liver function tests after 6 days of treatment. Also histopathological examination was performed using a histological scoring system. Results:The levels of MDA in the liver were significantly lower in the groups treated with dexamethasone compare to the group that underwent only biliary duct ligation (p=0,008) the same was seen in the group with chitosan (p=0,027) and chitosan with dexamethasone (p=0,014). Biliary duct ligation induced severe cholestasis, confirmed by altered liver function, portal and focal inflammation, necrosis and fibrosis. Conclusion: Dexamethasone and chitosan reversed all the biochemical parameters used to quantify liver pathophysiology and ameliorated histopathological changes previously induced by BDL. Findings of the present study suggest that this treatment may favor collagenolytic activity, an assumption supported by amelioration of necroinflammatory liver injury and fibrogenesis. dora.trip@yahoo.com
Clinical Cytology - Posters FINE NEEDLE ASPIRATION CYTOLOGY OF METASTATIC MERKEL CELL CARCINOMA Trutin Ostović K1 , Hariš V2 , Miletić Z1 , Lambaša S3 , Lajtman Z4 , Štoos-Veić T1 1Dubrava University Hospital, Department of clinical cytology and cytometry, Zagreb, Croatia 2 Dubrava University Hospital, Department of hematology, Zagreb, Croatia 3Dubrava University Hospital, Department of clinical and experimental pathology, Zagreb, Croatia 4Merkur University Hospital, Department of otorhinolaryngology and cervicofacial surgery, Zagreb, Croatia Aim: To present a case of metastatic Merkel cell carcinoma (MCC) diagnosed by fine needle aspiration cytology (FNAC), flow cytometric DNA analysis, pathohistology and electron microscopy. Case report: FNAC was performed of the nodule on left arm and neck 21 months since the primary MCC was diagnosed in 76-year-old female patient. We used a 23-gauge needle and a 20-ml syringe. One part of aspirate was used for cytological smears stained by Papenheim and Papanicolaou and the rest was used for DNA analysis by flow cytometry. The cytological features included increased cellularity, discohesive groups of malignant cells with large oval to irregular nuclei with moulding effect, inconspicuous micronucleoli and scanty cytoplasm. The tumour contained diploid peak with DNA index of 1.1and elevated S-phase fraction (21%). The cytological diagnosis of metastatic MCC was confirmed by histological one and by electron microscopy presented the pathognomonic features for MCC: dense-core neurosecretory granules with diameter of 100-250 nm surrounded by whorls of intermediate filaments. Conclusion: The MCC provides an enormous challenge for the morphologist because of a wide range of differential diagnosis. This case appears to be the first report of DNA analysis of a MCC from the aspirate. ktrutin@kbd.hr 145 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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