4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 80 Klinička citologija - Usmena predavanja FNA BASED DIAGNOSIS OF HEAD AND NECK LYMPHOMA Gjadrov Kuveždić K1 , Aurer I2 , Ries S1 , Sučić M1 , Marković-Glamočak M1 , Ilić I1 , Bašić-Kinda S2 , Radman I2 , Labar B2 1 Department of Pathology and Cytology, Zagreb University Hospital, Zagreb, Croatia 2Division of Hematology, Department of Internal Medicine, Zagreb University Hospital, Zagreb, Croatia Today the accepted World Health Organization (WHO) classification defines lymphoid diseases with combination of morphologic, immunologic, genotypic and molecular features, putting less emphasis on histopathology as a sole determination of lymphoma subtype. It enables FNA to become complementary morphologic method in lymphoma diagnostics. Aim: The aim of this study was to assess the efficacy of FNA in that region and to evaluate its sensitivity regarding lymphoma diagnostics. Patients and methods: in the study were included patients reffered to our laboratory from hematology department, in whom head and neck lymphadenopathia was found, and FNA preceded other procedure. Specimens were stained with MGG, and antibodies against CD3, CD5, CD10, CD20, CD23, CD43,CD68,CD45, CD15,CD30, ALK, and EMA were administered. Lymphomas were classified according to WHO classification. Results: In 248 patients 285 aspirations were performed. Diagnosis of lymphoma was given in 100 cases. Overall sensitivity was 90%, specificity 88%, predictive value of a positive result 97%, and predictive value of negative result 66%, with exact agreement in75% cases between cytomorphology and histopathology diagnoses. Conclusion: Based on our results FNA can be a method of choice, serving as a method complementary to histopathology in selected lymphoma cases. gjadrov@yahoo.co.uk
Clinical Cytology - Oral Presentations LIQUID-BASED CYTOLOGY - NEW POSSIBILITIES IN THE DIAGNOSIS OF CERVICAL LESIONS Jurič D1 , Mahovlić V1 , Rajhvajn S1 , Ovanin-Rakić A1 , Škopljanac-Mačina L1 , Barišić A1 , Šamija Projić I1 , Babić D2 , Suša M2 , Ćorušić A3 , Orešković S4 1 Department of Gynecologic Cytology 2 Department of Gynecologic and Perinatal Pathology 3 Department of Gynecologic Oncology 4 Department of Gynecology and Urogynecology, University Department of Gynecology and Obstetrics, Zagreb University Hospital Center, Zagreb, Croatia Introduction. Liquid-based cytology (LBC) enables the use of supplementary methods in the diagnosis and prognosis of cervical lesions. Aim. To analyse the correlation between p16INK4a immunoexpression in ThinPrep cervical cytologic samples and human papillomavirus (HPV) detection by polymerase chain reaction (PCR) from the same sample. Materials and methods. LBC-ThinPrep (Cytyc, USA) cervical cytology samples, prepared and stained by Papanicolaou method, were analysed using modified Bethesda cytologic classification named Zagreb 2002. A second ThinPrep slide, prepared from the same sample, was immunostained for p16INK4a using CINtec p16INK4a Cytology Kit (Dako- Cytomation, Denmark). Increased expression of the high-risk HPV E6 and E7 oncogenes results in a highly specific increase in p16 protein expression and overexpression of p16INK4a acts as a potential biomarker for cervical cancer progression from premalignant lesions. Brown nuclear and/or cytoplasmic staining of abnormal cells was considered a positive result. Residual material was used for 13 HR HPV-DNA detection by PCR based AMPLICOR HPV test ( Roche Molecular Systems). Results. A total of 120 Thin- Prep Pap tests with following cytological diagnosis: 17 within normal limits, 17 atypical squamous cell (ASC) (7 ASC of undetermined significance - ASCUS and 10 ASC of highgrade squamous intraepithelial lesions cannot be excluded – ASC-H ), 26 low-grade squamous intraepithelial lesions (LSIL) corresponding cervical intraepithelial neoplasia (CIN) I , 57 high-grade SIL (HSIL) i.e. 24 CIN II and 33 CIN III and 3 squamous cell carcinoma (SCC) were included in the study. All CIN III (n=33) and SCC (n=3) specimens expressed p16 immunoreactivity, whereas the HR HPV test was positive in 97% (32/33) of CIN III and 100% (3/3) of SCCs. The p16INK4a biomarker was positive in 87,5% (21/24) of CIN II and 69% (18/26) of CIN I, while the HR HPV was positive in 75% (18/24) of CIN II and 50% (13/26) of CIN I. In ASCUS cytology, p16 and HR HPV showed the same rate of positivity (28,5%; 2/7). Expression of p16 was detected in all cytological (10/10) ASC-H lesions, in contrast to HR HPV detected in only 20% (2/10) of the ASC-H cases. Conclusion. These data suggest the p16INK4a evaluation in ThinPrep cervical samples to be significantly associated with HR HPV testing by PCR in the same sample for a diagnosis of HSIL lesions and cervical carcinomas. A prospective study with longer follow up may clarify the predictive values in management of LSIL and ASC diagnosis. danijela_juric@yahoo.com 81 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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