4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije LYMPH NODE FINE NEEDLE ASPIRATION - SAMPLE ADEQUACY FLOW CYTOMETRY IMMUNOPHENOTYPING Švencbir V1 , Milas M2 , Anić V2 , Šiftar Z2 , Kardum Paro MM2 , Kardum-Skelin I2 1 Sestre Milosrdnice University Hospital, Zagreb, Croatia 2 Merkur University Hospital, Zagreb, Croatia In a modern clinical laboratory, lymphatic cell immunophenotyping by flow cytometry helps determine the diagnosis of clonal B-lymphocytes and thus discriminate benign from malignant lymphoproliferative diseases. The aim of the study was to assess adequacy and appropriateness of lymph node fine needle aspiration (FNA) for flow cytometry analysis in cases of benign and primary malignant disorders. The study was based on medical documentation, cytologic smear of FNA lymph node sample and flow cytometry immunophenotyping results. The study included 239 patients during the 2006-2007 period. According to cytologic test results, patients were classified into several groups: benign lymphoproliferative disease (22%), undefined monomorphous population of lymphatic cells (16%), B cell non-Hodgkin’s lyphoma (55%), and non-B cell non-Hodgkin’s lyphoma (5%). Lymphocytes were isolated from FNA lymph node samples using density gradient. The suspension of mononuclear cells was labeled by appropriate combination of negative control and monoclonal antibodies and then analyzed by flow cytometry. The acceptable minimum number of cells from lymph node sample as representative for flow cytometry analysis was determined by maximal control of electronic gates around the specific cell population. In the study material, 85% of samples contained an adequate cell quantity for analysis, in 12% of samples the number of cells was only adequate to determine clonality, whereas in 3% of samples the number of cells was inadequate for any analysis. Although cell adequacy for analysis varied among groups, there was no statistically significant difference in the adequacy of samples obtained from patients with benign lymphoproliferative diseases (87%), monomorphous population of lymphatic cells (76%), B cell non-Hodgkin’s lymphoma (89%) and non-B cell lymphoma (69%). In conclusion, cytologic diagnosis of lymphoproliferative disease is a simple and reliable diagnosis in both separation of benign from primary lymphoproliferative diseases (lymphoma) and secondary malignancies, as well as in tissue typing and subtyping of malignant lymphoma. Since FNA provides enough cells for flow cytometry analysis, these two diagnostic methods improve diagnostic accuracy and safety viktorija_svencbir@net.hr 184 Citotehnologija - Posteri
indeks autora authors’ index 185 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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4. Hrvatski kongres kliniËke citol
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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