4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije 86 Klinička citologija - Usmena predavanja ESTIMATING CLINICAL OUTCOME OF HPV INDUCED CERVICAL LESION BY COMBINATION OF CAPSID PROTEIN L1 AND P16INK4a PROTEIN DETECTION Krivak Bolanča I, Šentija K, Katalenić Simon S, Kukura V*, Vraneš J** Laboratory for Clinical Cytology and Genetics *Department of Gynaecology and Obstetrics “Merkur” University Hospital, Zagreb ** “Dr.Andrija Stampar” Institute of Public Health, Zagreb, Croatia The aim of this study was to investigate whether is possible to predict clinical outcome of cervical lesion by immunoassaying performed on cervical smears. During the two year study period the cervical smears of 81 patients were collected. All patients were tested for human papillomavirus (HPV) infections using Amplycor HPV test. Sixty-six of them were tested as positive for high risk types (hrHPV) and squamous intraepithelial lesion, and in those patients repeated cervical smears were taken every six months. The rest were hrHPV negative patients with normal smears which were used as a negative control in immunoassays with HPV L1 and p16INK4a antibodies. The results of p16INK4a staining in 66 hrHPV positive patients showed impairment of the cervical lesion in 22 (33.3%) and unchanged cytological finding in 21 (31.9%) p16INK4a positive patients, respectively, while improving of cytological finding was seen only in three (4.5%) p16INK4a positive patients. On the contrary, impairment of cytological finding was not seen in p16INK4a negative patients, while in 17 out of 20 patients from that group improving or normalisation of cytological finding were detected (p
Clinical Cytology - Oral Presentations ALCOHOL SCLEROSING OVARIAN CYSTIC LESIONS, 20 YEARS EXPERIENCE Kukura V, Krivak Bolanča I*, Šentija K*, Katalenić Simon S* Department of Gynecology and Obstetrics *Laboratory for Cytology and Clinical Genetics “Mekur“ University Hospital, Zagreb, Croatia Objectives: The purpose of the study is a technique of punction and conservative treatment of cystic ovarian lesions. Methods: The cyst should be unilocular, sonolucent, with a smooth inner wall of capsule, without septa and without neovasculariation on transvaginal color Doppler. CA-125 levels must be lower than 35 U/ml. The capsule of the cyst was punctured with a 18 gauge needle under the control of 5 MHz transvaginal probe. Cyst fluid was sent for cytologic examination. After complete emptyng of the cyst, we injected sterile 95% ethanol in the 50-75% of the evacuated liquor amount. The alcohol remain in the cyst from 5 to 20 minutes and was then aspirated completely. Results: We punctured 366 patients with ovarian cyst volume between 40 and 300 ml in the age from 18 to 65. Patients were monitored for 24 hours and follow-up examinations are 3, 6 and 12 months after the procedure. Intensive pelvic pain had 8,1% and relapse appeared in 8,2% of the patients. Three cysts were ruptured (0,8%) and alcohol split in the Douglas cavity. Conclusion: Technique of punction is simple and easily performed. Method of treating by 95% alcohol showed good results. Relapse we treated by laparoscopy or laparotomy. vlastimir.kukura@zg.t-com.hr METASTASES ON RARE LOCATIONS AS INITIAL MANIFESTATIONS OF NON-SMALL CELL LUNG CARCINOMA: TWO CASE REPORTS Lozić AAB, Besser Silconi Ž, Mišljenović N Department of Cytology, Pula General Hospital, Pula, Croatia The non-small cell carcinoma of the lung is a malignant epithelial tumor slow in growth but still metastasizing out of its site. When the primary tumor treatment starts, about 60% of patients already have some kind of malignant cell spreading. Metastases to hand bones and skeletal muscles are very rare (metastatic hand lesions represent 0,1% of all osseous metastases while metastases to muscles represent from 0,8 to 16% incidence in autopsy series) but the metastatic involvement of these sites usually indicate that metastatic disease has already spread. Fine needle aspiration cytology has an important task to give accurate diagnosis or at least diagnosis of suspicion and thus to set the guidelines to a clinician for the further specific and cost-effective treatment. We will show two cases where the metastases of non-small cell carcinoma of the lung were the first signs of the disease in uncommon body parts: a man with the metastasis to distal phalanx of the right hand thumb and a woman with nodal metastasis to the right gluteal muscle and subcutaneous tissue near muscle, so we have to pay our attention to the potential development of such lesions in uncommon body parts. alex_lozic@yahoo.co.uk 87 4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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