4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

Cytotechnology - Oral Presentations
NUMBER OF COUNTING CELLS AND CYTOSPINS SELECTION INFLUENCES ON
BRONCHOALVEOLAR LAVAGE CELL PROFILES
Harabajsa S, Martinčić J, Peharec I, Popek B, Šušković- Medved S, Zadražil B,
Smojver-Ježek S
University Hospital for Lung Diseases Jordanovac, Zagreb, Croatia
BAL fluid cells count provides information about presence or absence of interstitial lung
diseases. BAL fluid samples were taken from 50 patients hospitalized in University Hospital
for Lung Diseases Jordanovac in Zagreb, Croatia. The samples of BAL fluid were
prepared by cytocentrifuge. From each sample two cytospin were selected (C1 and C2)
and after determing adequacy, counted up to 200 and 400 cells. After air-drying, samples
were stained according to May Grünwald Giemsa (MGG). Cells were counted by
light microscope at magnification of 400 x. Obtained results were analyzed in Statistics
version 6 and Med Calc. Results for bronchial epithelial cells, alveolar macrophages,
lymphocytes and neutrophilic granulocytes showed insignificant statistical differences
between groups (p>0.05). Eosinophils percentages showed borderline insignificant statistical
difference between groups of these cells (p=0.052). As it was exemplificated, the
percentages of differentiated cells do not significant differ according to differentiation on
200 and 400 cells and cytospin selection.
suzana.harabajsa@zg.t-com.hr
ERYTHROCYTE MORPHOLOGY IN MALIGNANT URINE SAMPLES
Knežević G, Parigros K, Milas M, Šušterčić D, Kardum-Skelin I
Merkur University Hospital, Zagreb, Croatia
The aim of the study was to show the origin of erythrocytes in malignant urine sample,
assuming that a finding of 80% and more of dysmorphic erythrocytes indicated the
bleeding to derive from upper urinary tract, and 80% and more of smooth erythrocytes
indicated them to derive from lower urine tract; in urine samples without significant
origin of bleeding there were 20%-80% mixed results with both dysmorphic and isomorphic
erythrocytes. Data were collected from the findings recorded in malignant urine
samples received. Samples were fresh native urine sediment contrast stained with 0.1%
Safranin solution and analyzed under light microscope (X40). Out of 72 patients with
malignant cells detected in urine, the origin of erythrocytes was identified in 25 patients
(nine female and 16 male) through 190 samples (approximately 4 samples per patient).
A mixed origin of erythrocytes was identified in 70 (36.9%) samples, 55 (28.9%) samples
did not have enough erythrocytes to define their origin, dysmorphic erythrocytes were
found in 53 (27.9%) samples, and isomorphic erythrocytes in 12 (6.3%) samples. In conclusion,
there was no specific connection between malignant cell findings in urine and
origin of erythrocytes. However, the high presence of mixed erythrocyte origin in malignant
samples may suggest that the existence of a malignant process and renal disease
should be taken in consideration.
gordana.knezevic1@zg.t-com.hr
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4 th Croatian Congress of Clinical Cytology / 1 st Croatian Symposium of Analytical Cytology / 2 nd Croatian Symposium of Cytotechnology