4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije MICROSCOPIC IDENTIFICATION OF PARASITES IN CYTOLOGICAL PREPARATIONS - THE „GOLD STANDARD“ IN DIAGNOSTICS OF VISCERAL LEISHMANIASIS - A CASE REPORT Stupnišek M, Prstec Z, Vujević D, Čulig Z, Puljiz I, Lukas D, Begovac J. University Hospital for Infectious Diseases “Dr. Fran Mihaljević”, Zagreb, Croatia Visceral leishmaniasis (VL) is an infectious, potentially fatal, systemic disease characterized by fever, fatigue, splenomegaly, hepatomegaly, progressive anemia, pancytopenia and hypergammaglobulinemia. VL is caused by protozoan parasites that belong to the genus Leishmania and are transmitted to mammals by the bite of female sandfly. The causative agent can be detected by: microscopic identification of parasites in stained cytological preparations (usually from bone-marrow, spleen, lymph-node); cultivation; serological and molecular methods. VL is spread worldwide. In Croatia, VL occurs rarely (around 3-5 cases per year) usually in persons who reside or live in middle and southern Dalmatia. Of particular concern is the emerging problem of VL/HIV co-infection, especially in southern Europe. The clinical presentations as well as organ involvement of VL are often atypical in HIV+ patients. We report a rare case of VL with pulmonary localization in a 57-year-old HIV+ patient. Leishmania amastigotes were detected in bonemarrow aspirates in several instances. The patient was re-admitted to the Hospital due to high fever and the presence of pulmonary infiltrate. Bronchoscopy was performed and bronchoalveolar lavage (BAL) specimen collected. The preparations were stained by May-Grünwald-Giemsa and Diff-Quick technique and observed by light microscope. Leishmania amastigotes were found. In samples of BAL obtained during subsequent bronchoscopy, planocellular carcinoma was also detected. Conclusions: In patients with VL, especially immunocompromised, atypical clinical presentations and unusual localizations of disease are commonly found, but pulmonary localization is rarely than others. Microscopic identification of parasites in cytological preparations presents the „gold standard“ in diagnostics of VL, which primarily requires well educated laboratory personnel. stupnisek@gmail.com 180 Citotehnologija - Usmena predavanja
Cytotechnology - Posters ANALYSIS OF ABNORMAL PAP TEST FINDINGS OF URBAN, RURAL AND INSULAR AREA Bašić D, Markanjević T University Hospital Center Rijeka, Croatia Objective: Comparison of abnormal Pap test findings of women sampled in urban, rural and insular area.Methods: In the Department of Gynaecological cytology, University Department of Gynecology and Obstetrics, University Hospital Center Rijeka, Croatia, Pap tests from almost entire County of Primorje-Gorski kotar and a part of County of Lika-Senj are analyzed. In 2008 36,153 Pap tests have been analyzed. In the research we analyzed the findings of Pap tests choosing one medical clinic in urban, one in rural and one in insular area. The frequency of abnormal findings has been analyzed as well as distribution according to age and the frequency of abnormal findings of squamous and glandural epithelium. Statistical significance between the groups has been analyzed by using chi-square test.Results: From total of 4492 samples analyzed we found 279 (6,2%) abnormal Pap test, out of which 279 (93,5%) of squamous and 18 (6,5%) of glandular epithelium origin. In the urban area 125 (5,7%) abnormal Pap test were found, out of which 121 (96,8%) of squamous epithelium and 4 (3,2%) of glandural epithelium. In the rural area 50 (6,2%) abnormal Pap test were found, out of which 44 (88%) of squamous epithelium and 6 (12%) of glandural epithelium. In the insular area 104 (7%) abnormal Pap test were found, out of which 96 (96%) of squamous epithelium and 8 (8%) of glandural epithelium. By distribution according to age, in urban area 50 (40%) abnormal findings were found among women up to age 30, 54 (43%) among women from age 30 to 50 and 21 (17%) among women above age 50. In the rural area there were 15 (30%) abnormal Pap test among women up to age 30 and 25 (50%) abnormal Pap test among women from age 30 to 50 and 10 (20%) among women above age 50. In the insular area there were 48 (46%) abnormal Pap test among women up to age 30, 39 (38%) among women from age 30 to 50 and 17 (16%) among women above age 50. By statistical analysis a statistically significant higher values of frequency of abnormal findings of glandular epithelium was detected in rural area in comparison to women of urban area (p
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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