4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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4. Hrvatski kongres kliniËke citologije / 1. Hrvatski simpozij analitiËke citologije / 2. Hrvatski simpozij citotehnologije BIOMED-2 PCR DETECTION OF IGH/BCL2 REARRANGEMENT IN FOLLICULAR LYMPHOMA USING DNA OBTAINED FROM ARCHIVED CYTOLOGICAL SMEARS: AN OPTION FOR MONITORING Štoos-Veić T, Livun A, Ajduković R, Pejša V, Jakšić O, Kušec R Dubrava University Hospital, Zagreb, Croatia Aim. To determine the rate of PCR detection of IgH/BCL2 in DNA samples isolated from archival cytological slides of lymph node aspirates, bone marrow and/or peripheral blood (PB) obtained from patients with histologicaly confirmed follicular lymphoma using primers and protocol proposed by BIOMED-2 consortium. We also compared molecular with cytomorphological findings in bone marrow/ peripheral blood and tested the clinical value of IgH/BCL2 molecular marker in monitoring minimal residual disease (MRD). According to WHO classification follicular lymphoma is a neoplasm composed of follicle centre B-cells, which usually has at least a partially follicular pattern. Bone marrow (BM) infiltration by lymphoma occurs in 40-70% of cases at the time of diagnosis. The characteristic genetic aberration of follicular lymphoma is t(14;18)(q32;q21) with transposition of BCL2 oncogene to the regulatory region of immunoglobulin heavy chain gene IgH. Methods. DNA was isolated from archival cytological slides obtained by fine needle aspiration of lymph nodes from 19 patients and PCR based detection of IgH/ BCL2 was performed. Results. Fusion oncogene was detected in 10 of 19 patients (52%). For patients with IgH/BCL2 positive lymph nodes molecular test found BM infiltration in 5 cases (83%), while cytomorphology detected infiltration in three of eight cases (37%) available for comparison. Conclusions. May-Grünwald Giemsa stained cytological smears can be used for PCR-based ancillary methods and the rate of detection of IgH/ BCL2 rearrangement is similar to results reported for paraffin-embedded tissues. For cases with detectable baseline molecular marker, PCR is a highly suitable method for detection of bone marrow involvement and monitoring MRD. tveic@kbd.hr 160 Analitička citologija - Plenarna i pozvana predavanja
Analytical Cytology - Plenary and Invited Lectures FLOW CYTOMETRY AND DIAGNOSTIC MONITORING OF HIV-INFECTED PATIENTS Židovec Lepej S University Hospital for Infectious Diseases Zagreb, Croatia Aim. Quantification of CD4+ T-cells in the peripheral blood of infected patients is an important diagnostic parameter for the classification of patients and monitoring of immune reconstitution. Immune hyperactivation also plays an important role in the pathogenesis of HIV-disease. The aim of this study was to compare the expression of activation marker CD38 on CD8+ T-cells in HIV-infected patients and controls as well as in treated and untreated HIV-patients. Methods. Expression of CD38 on CD8+ T-cells was analyzed by flow cytometry (Cytomics FC500, Beckman Coulter, USA). Immune status of HIV-patients was determined by using Centers for Disease Control and Prevention panel for HIV/AIDS recommended in 2003 (5-color staining). Results. Expression of CD38 on CD8+ T-cells in untreated HIV-infected patients (n=47, median 34.2%, range 17.9- 43.2%) was significantly higher compared to treated patients (n=50, median 17.2%, range 10.4-20.9%, p
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Final programme and abstract book
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UTORAK, 13.10.2009. SRIJEDA, 14.10.
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4. Hrvatski kongres kliniËke citologije 4th Croatian Congress ... - Penta

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