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ings (aromatic and nonaromatic) . The structure of these <strong>com</strong>plex mixtures was<br />

not elucidated at this time, but could be studied in the future.<br />

RESULTS<br />

Results are presented in Tables 1, 2, and 3. The weight of the <strong>com</strong>pounds<br />

AVSP (before saponification) and APSP (after saponification) are indicated.<br />

The value of the ratio AVSP/APSP is characteristic of the capacity of the<br />

medium to be biodegraded. A value close to 1 shows poor bacterial activity.<br />

As the value increases, better biodegradation is indicated. This fact is due<br />

to the functionalized intermediate <strong>com</strong>pounds made by the bacteria and extract<br />

in basic medium.<br />

The fraction FB is constituted by unsaponif iable <strong>com</strong>pounds. Comparison<br />

of FA and FB is not interesting. Only the <strong>com</strong>parison of FB values for<br />

different sampling times is relevant. When biodegradation increases (less<br />

linear hydrocarbons) , the FB fraction decreases. At Al and A2 station (in<br />

very polluted zones) , the concentrations of hydrocarbons (linear or<br />

substituted) were about null in 1980, but <strong>com</strong>plex organic <strong>com</strong>pounds of heavy<br />

molecular weight, which are extractable with hexane (since present in the FA<br />

fraction) , remain in the sediment. The molecular structure of these <strong>com</strong>pounds<br />

has not been established (the pet role urn- type asphaltenes are not extracted at<br />

the very beginning by pentane) .<br />

Station C.<br />

CONCLUSIONS<br />

In 1980, in spite of the presence of 0.54 g in the FA fraction, its<br />

chromatogram was no longer characteristic of a petroleum-polluted zone (C17,<br />

C18, C19 predominant). However, light petroleum pollution is indicated at 20<br />

cm below the surface.<br />

Station B_<br />

In 1980, various deposits did not allow correct sampling; therefore, only<br />

1978 and 1979 values were determined. In 1979, there seemed to an indication<br />

of return to normal state, especially at the rhizome level (bottom) .<br />

Station A.<br />

In 1980, half of the hydrocarbons of 1979 remained. This fraction did<br />

not show the presence (by chromatogram) of linear or substituted hydrocarbons;<br />

however, its weight cannot be explained only by the unbiodegradable cyclanes<br />

or aromatics (33 g) .<br />

104

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