03.07.2013 Views

download 21.6mb - Oil-Spill-Info.com

download 21.6mb - Oil-Spill-Info.com

download 21.6mb - Oil-Spill-Info.com

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

clumps in the ovary to foci in the leydig tissue of the testes. Nineteen<br />

(86.4%) of the cases were in female gonads. Reproductive stages<br />

varied from undeveloped to spawned. Three of the cases were found in<br />

testes.<br />

In the general and focal leucocytoses discussed above, the difference<br />

between the two was in the extent (small area, tight clump vs.<br />

general dispersion over several follicles) of inflammation, but this<br />

was sometimes difficult to ascertain and the two may blend together.<br />

Necrotic appearing areas were noted in 3.1% (4 of 130) of the gonads<br />

examined. These areas were characterized by cellular debris, degenerating<br />

ova, and leucocytosis.<br />

Sporozoa were present in 4.6% (6 of 130) of the specimens examined.<br />

Sporozoans were spherical, densely staining, and were embedded in the<br />

gonadal tissue. The specimens appeared to be surrounded by a small lysed<br />

"halo" area.<br />

5. Gill . - Gills from 134 C. gigas were examined for pathologies.<br />

Generally, three pieces of gill (consisting of both lamellae) were<br />

dissected from one side and oriented (when possible) to give both longitudinal<br />

and transverse sections.<br />

Histopathologies were noted in 31.3% (42 of 134) of the gills<br />

examined. There were a total of 49 cases of the six pathology types<br />

described below. Forty-three (87.8%) were apparently not symbiotic or<br />

related to a symbiotic condition.<br />

. Abnormally high numbers of eosinophilic leucocytes were present in<br />

31.3% (42 of 134) of the gills examined. In most incidences, the leucocytes<br />

were dispersed throughout several plica, but four cases appeared<br />

to be more focally organized in one or two plica.<br />

Amoebae were noted in the gills of one C. gigas. The infection<br />

appeared to be light as only two amoebae were found. The amoebae were<br />

circular in outline with a hyaline cytoplasm. The nucleus occupied<br />

approximately one-third of the cell. A small, spherical inclusion<br />

body was adjacent to the nucleus.<br />

The gills of four (3%) specimens examined harbored ciliates in<br />

their water tubules. Ciliates were somewhat crescent-shaped with tufts<br />

of stout cilia extending downward from the two tips. The arms of the<br />

crescent were sometimes turned inward so that the tips of the cilia were<br />

touching, giving a partially hollow, circular shape to the ciliate. Two<br />

282

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!