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RESULTS<br />

Tissues from 134 specimens of Cvassostvea gigas from four sites were<br />

examined for histopathologies over five sampling trips. From the specimens<br />

collected, tissue samples of 131 adductor muscles, 127 stomach/<br />

intestines, 129 digestive glands, 130 gonads, 134 gills, and 130 mantles<br />

were examined for a total of 781 tissues out of a possible 804.<br />

A total of nine types of pathologies were found with an incidence<br />

of 241 occurrences (Table 1). Five-hundred and ninety of the 781 (75.6%)<br />

tissues examined were free of pathologies; or, 191 of the 781 (24.3%)<br />

tissues examined bore one or more pathologies. Of the 241 pathologies<br />

found, 77 (32.0%) were various types of symbioses, while 164 (68.0%)<br />

cases apparently were not correlated with symbioses. Table 2 summarizes<br />

the distribution of pathologies among the tissues examined. Adductor<br />

muscle had the lowest incidence (3.8%). Digestive gland tissue had the<br />

highest incidence (23.9%) followed by gill (22.0%), mantle (21.4%), gut<br />

(17%), and gonad (11.9%). The number of tissues with pathologies was<br />

nearly evenly distributed among the collecting sites. Oysters from<br />

reference stations had a higher incidence of lesions, particularly in<br />

gonad and gill, than oysters from oil-polluted sites. Thirty percent of<br />

the oyster tissues from both Aber Wrac'h and Aber Benoit bore one or more<br />

pathologies. Forty percent of the tissues from Rade de Brest and lie<br />

Tudy <strong>com</strong>bined contained one or more pathologies.<br />

Overall, mantle bore the lowest number of pathology types (3) while<br />

digestive gland contained the most types of pathologies (9) followed by<br />

gut (7), gill (6), gonad (5), and muscle (4).<br />

Pathologies and their distributions among organs and sites are<br />

described below.<br />

1. Muscle . - Muscle tissues were examined from 131 C. gigas.<br />

Samples for microscopic examination were dissected from the adductor<br />

muscle and both fast and catch muscles were examined when possible.<br />

Generally, two tissue samples were taken from each muscle and oriented<br />

to give both longitudinal and cross sections.<br />

Histopathologies occurred in 4.6% (6 of 131) of the muscle samples<br />

examined. There were a total of 9 incidences of. the three pathologies<br />

described below. Muscle from reference stations contained the widest<br />

variety of pathologies. No pathologies were found in muscles from Aber<br />

Benoit.<br />

273

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