1 - Instituto de Biologia da UFRJ

More documents

Recommendations

Info

Bacteria a Archaea b c Microeukaryotes Figure 5. Rarefaction curves calculated using DOTUR 0.03 . The partial sequences of microbial SSU rRNA genes from Antarctic oil-contaminated and oiluncontaminated soils (mixture 1:1:1 and 1:1 of samples of oil-polluted and oil-unpolluted areas of the Brazilian Antarctic Station, respectively) was used. a, b, c) curves of each library of each bacterial, archaeal and microeukaryotic domains respectively. Discussion and Conclusion The recalcitrance of hydrocarbons in cold soils may be due characteristics as: low temperatures (Haider, 1999), lack of final electron acceptors (especially oxygen) (Johnsen et al., 2005), low nutrient concentrations or availability (especially N and P) (Aislabie et al., 2006), or the absence of microorganisms capable of using hydrocarbons as C source (Johnsen et al., 2005; Huesemann et al., 2002). Two of the cited factors must be contributing to the hydrocarbon recalcitrance in the soil of the Brazilian Antarctic Station: the nutrient content, especially Nitrogen, considering the negligible amounts of this element in soil; and the lack of oxygen as electron acceptor, considering that the polluted area remains under constant water logging and soil compression due the thaw of the upstream snow and the constant traffic of vehicles, respectively, which decreases the oxygen diffusion. The absence of degrading microorganisms not seems to be one recalcitrance factor considering the positive results obtained in the PCR of the tested genes involved in the hydrocarbon degradation. These results indicate that the microbial community of the oil-polluted soil of the Antarctic Brazilian Station is able to perform metabolic pathways of the hydrocarbon degradation. An indication of the microorganisms participation in the hydrocarbon depletion via degradation is the dose-response effect observed with the application of the Nitrogen. The application of N at a rate between 125 and 250 mg.kg –1 seems to be sufficient to promote further 192 | Annual Activity Report 2011
degradation of the hydrocarbons regardless the effect of the aeration. The present data provides information that allows us to propose the appropriate methodology that can be applied in the area of the Brazilian Antarctic Station for the bioremediation process. In addition, it provides information that allows us to propose an appropriate action plan using better recommended materials (e.g. type and dose of fertilizer; stock of a consortia of degraders strains) that will be available for immediate use in the case of new contaminations due to fuel spills in the Brazilian Antarctic Station. Acknowledgements This work integrates the National Institute of Science and Technology Antarctic Environmental Research (INCT- APA) that receives scientific and financial support from the National Council for Research and Development (CNPq process: n° 574018/2008-5) and Carlos Chagas Research Support Foundation of the State of Rio de Janeiro (FAPERJ n° E-16/170.023/2008). The authors also acknowledge the support of the Brazilian Ministries of Science, Technology and Innovation (MCTI), of Environment (MMA) and Inter- Ministry Commission for Sea Resources (CIRM). References Aislabie, J.; Balks, M.R.; Foght, J. & Waterhouse, E.J. (2004). Hydrocarbon spills on Antarctic soils: effect and management. Environmental Science & Technology, 38(5): 1265-1274. Aislabie, J.; Sauld, D.J. & Foght, J.M. (2006). Bioremediation of hydrocarbon-contaminated polar soils. Extremophiles, 10: 171-179. Alexander, M. (ed.) (1994). Biodegradation and Bioremediation. San Diego: Academic Press Inc. Delille, D.; Coulon, F. & Pelletier, E. (2004). Biostimulation of natural microbial assemblages in oil-amended vegetated and desert sub-Antarctic soils. Microbial Ecology, 47:407-415. Haider, K. (1999). Microbe-soil-organic contaminant interactions. In: Adriano, D.C.; Bollag, J.M.; Frankenberger, W.T. & Sims, R.C. (Eds.). Bioremediation of contaminated soils. Madison: ASA/CSSA/SSSA. p. 33-51. Huesemann, M.H.; Hausmann, T.S. & Fortman, T.J. (2002). Microbial factors rather than bioavailability limit the rate and extent of PAH biodegradation in aged crude oil contaminated model soils. Bioremediation Journal, 6(4): 321-336. Johnsen, A.R.; Wick, L.Y. & Harms, H. (2005). Principles of microbial PAH-degradation in soil. Environmental Pollution, 133(1): 71-84. Luz, A.P.; Pellizari, V.H.; Whyte, L.G. & Greer, C.W. (2004). A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil. Canadian Journal of Microbiology, 50: 323-333. Luz, A.P.; Ciapina, E.M.P.; Gamba, R.C.; Lauretto, M.S.; Farias, E.W.C.; Bicego, M.C.; Taniguchi, S.; Montone, R.C. & Pellizari, V.H. (2006). Potential for bioremediation of hydrocarbon polluted soils in the Maritme Antarctic. Antarctic Science, 18(3): 335-343. Reinhardt, S.B. & Van Vleet, E.S. (1986). Hydrocarbons of Antarctic midwater organisms. Polar Biology, 6:47–51. Saul, D.J.; Aislabie, J.M.; Brown, C.E.; Harris, L. & Foght, J.M. (2005). Hydrocarbon contamination changes the bacterial diversity of soil from around Scott Base, Antarctica. FEMS Microbiology Ecology, 53: 141-155. Science Highlights - Thematic Area 4 | 193
Page 2 and 3:
Annual Activity Report 2011 Expedie
Page 4 and 5:
Cataloguing Card I59a Annual Activi
Page 6 and 7:
PRESENTATION National Institute of
Page 8 and 9:
6 | Annual Activity Report 2011
Page 10 and 11:
Thematic Research Areas The Researc
Page 12 and 13:
introduction Advances In Brazilian
Page 14:
of soil contamination. At present,
Page 18 and 19:
THEMATIC AREA 1 ANTARCTIC ATMOSPHER
Page 20 and 21:
One of the most important propertie
Page 22 and 23:
1 ATMOSPHERIC CHANGES OBSERVED IN A
Page 24 and 25:
the F2 layer critical frequency par
Page 26 and 27:
with the Gleissberg cycle (~90 year
Page 28 and 29:
2 MESOSPHERIC GRAVITY WAVES OBSERVE
Page 30 and 31:
of 2011 (now under analysis). The h
Page 32 and 33:
3 SYNOPTIC WEATHER SYSTEM ASSOCIATE
Page 34 and 35:
a b c Figure 2. Wind field daily av
Page 36 and 37:
4 INFLUENCE OF THE ANTARCTIC OZONE
Page 38 and 39:
and New Zealand (Brinksma et al., 1
Page 40 and 41:
of Science, Technology and Innovati
Page 42 and 43:
a b Figure 1. South tower of the Br
Page 44 and 45:
a b c d e f g Figure 3. Diurnal var
Page 46 and 47:
and infrared gas analyzer were setu
Page 48 and 49:
THEMATIC AREA 2 IMPACT OF GLOBAL CH
Page 50 and 51:
were widespread, reaching latitudes
Page 52 and 53:
GPS to reference the points in an a
Page 54 and 55:
Figure 2 can suggest that the 6 pat
Page 56 and 57:
Materials and Methods Phytossociolo
Page 58 and 59:
References Bednarek-Ochyra, H.; Van
Page 60 and 61:
level and parent material); vegetat
Page 62 and 63:
the soil organic matter levels. The
Page 64 and 65:
4 CONSERVATION STATUS OF MOSS SPECI
Page 66 and 67:
Figure 1. The most frequent moss fo
Page 68 and 69:
Olech, M. (1996). Human impact on t
Page 70 and 71:
of apothecia or perithecia) were ma
Page 72 and 73:
Acknowledgements This work integrat
Page 74 and 75:
(Figure 1). There were found two sp
Page 76 and 77:
completely smooth hymenophore (Ager
Page 78 and 79:
Results The average SOI presented a
Page 80 and 81:
Table 1. ANCOVA results of demograp
Page 82 and 83:
8 RESPONSES OF AN ANTARCTIC KELP GU
Page 84 and 85:
Figure 2. Average number of Kelp Gu
Page 86 and 87:
9 Population fluctuation of Pygosce
Page 88 and 89:
winter habitat selection (Trivelpie
Page 90 and 91:
10 FORAGING DISTRIBUTION OF AN ANTA
Page 92 and 93:
December and January) with a Repeat
Page 94 and 95:
THEMATIC AREA 3 Impact of human act
Page 96 and 97:
these two types of sewage markers,
Page 98 and 99:
1 TEMPERATURE, SALINITY, PH, DISSOL
Page 100 and 101:
Results In Table 1 we show the ocea
Page 102 and 103:
Robakiewicz, M. & Rakusa-Suszczewsk
Page 104 and 105:
we show the results from early summ
Page 106 and 107:
a b c d Figure 2. Variations at dif
Page 108 and 109:
3 SUMMER VARIATION OF ZOOPLANKTON C
Page 110 and 111:
a b Figure 2. Mean numbers of holop
Page 112 and 113:
Discussion Larval forms are common
Page 114 and 115:
4 ASSESSMENT OF FAECAL POLLUTION IN
Page 116 and 117:
Presence of C. perfringens in DCRM
Page 118 and 119:
Figure 3. Most probable number and
Page 120 and 121:
Leclerc, H.; Mossel, D. A.; Trinel.
Page 122 and 123:
Figure 1. Map of the study region w
Page 124 and 125:
Fourteen n-alkanols were identified
Page 126 and 127:
Acknowledgements This work integrat
Page 128 and 129:
treatment procedures that clean the
Page 130 and 131:
Figure 2. Fecal sterols in marine s
Page 132 and 133:
7 FRACTIONATION OF TRACE METALS AND
Page 134 and 135:
a b Figure 1. Trace element mobile
Page 136 and 137:
the National Council for Research a
Page 138 and 139:
of humans (Bargagli et al., 1998).
Page 140 and 141:
Farías, S.; Smichowski, P.; Velez,
Page 142 and 143:
Materials and Methods In Admiralty
Page 144 and 145: stations. This alteration acts as a
Page 146 and 147: 10 A baseline studies on plasmatic
Page 148 and 149: higher than ECO and PP, where as to
Page 150 and 151: 11 Effect of diesel oil on gill enz
Page 152 and 153: Figure 1. Activity of enzymes hexok
Page 154 and 155: Regoli, F.; Principato, G.B.; Berto
Page 156 and 157: (Steneck et al., 2002), consisting
Page 158 and 159: Munnidae, Plakarthidae, Jaeropsidae
Page 160 and 161: 13 TRACKING NON-NATIVE SPECIES IN T
Page 162 and 163: Table 1. Percentage of records in t
Page 164 and 165: References Carlton, J.T. (2009). De
Page 166 and 167: liverworts, lichens, algaes (Smykla
Page 168 and 169: a b c d Figure 2. Taxonomic details
Page 170 and 171: McInnes, S.J.; Chown, S.L.; Dartnal
Page 172 and 173: THEMATIC AREA 4 ENVIRONMENTAL MANAG
Page 174 and 175: 1 RELATIONSHIP BETWEEN NOISE AND PS
Page 176 and 177: a b Figure 1. EACF acoustic mapping
Page 178 and 179: increases to 55 dB (A) the speech r
Page 180 and 181: 2 ANALYSIS OF INDOOR ALDEHYDES IN T
Page 182 and 183: filtered and stored in vials, below
Page 184 and 185: formaldehyde concentration too. Des
Page 186 and 187: 3 RESULTS OF THE INTERNAL AUDIT IN
Page 188 and 189: Figure 1. Requirements of ISO 14.00
Page 190 and 191: 4 BIOREMEDIATION OF THE DIESEL-CONT
Page 192 and 193: Figure 1. Soil sampling points in t
Page 196 and 197: EDUCATION AND OUTREACH ACTIVITIES D
Page 198 and 199: Informative and educational materia
Page 200 and 201: 6. T-shirts: for each exhibition, t
Page 202 and 203: FACTS AND FIGURES Human Resources:
Page 204 and 205: publications Papers Bageston, J.V.;
Page 206 and 207: Fanticele, F. B. Avaliação de con
Page 208 and 209: e-mails inct - apa reserach team Th
Page 210 and 211: Dr. Márcia Caruso Bícego (IOUSP)
Page 212: Tecnologista Heber Passos (INPE/INC
Page 215: Instituto Nacional de Ciência e Te
show all

1 - Instituto de Biologia da UFRJ

Create successful ePaper yourself

Delete template?

Save as template?