Abai, MR

6th International Congress of DipterologyKey Words: aNDVI, cecidomyiid gall, CHB, NDVI, NPCI, oval-pointed,photosynthetic reflectance properties, PRI, R/G, SIPI∗∗∗∗∗∗∗∗∗∗A Close Correlation among Histone Acetylation, hsp70 GeneExpression and Longevity in Drosophila melanogasterHui, S., Z. Yanmei & H. BaiquThe Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024,P.R. ChinaThe heat shock proteins (Hsps) play a positive role in lifespandetermination, and histone acetylation has been shown to be involved intranscription of hsp genes in Drosophila. To further determine if hsp70expression is correlated with lifespan, and if histone acetylationparticipates in this process, RNA levels for hsp70 were analyzedthroughout the lifespan in the long-lived and short-lived iso-female lines.The results showed that hsp70 RNA levels were higher in long-lived linethan in short-lived line and that the longlived flies responded more rapidlyto heat but were more tolerant to high temperature. Moreover, weinvestigated the influences of histone acetylation modification on longevityand on hsp gene expression by using histone deacetylase (HDAC)inhibitors. The results demonstrated that both inhibitors were able toextend the lifespan and promote hsp70 expression. However,Themechanisms of their actions may vary with the genetic background. Inaddition, we showed that HDAC inhibitors caused the hyperacetylation ofcore histone H3 at the promoter and the transcribing regions of hsp70 gene.Key Words: hsp70, lifespan, histone acetylation, histone, deacetylaseinhibitor, Drosophila melanogaster120