Abai, MR

6th International Congress of DipterologySporogony cycle of Plasmodium vivax in Anopheles stephensimysorensis and effects of inhibitor carbohydrates on thedevelopment of parasiteBasseri, H.R. (1), S. Doosti, M. Nateghpour & K. Akbarzadeh(1) Department of Medical Entomology and Vector Control, School of Public Health,Tehran University of Medical SciencesThe aim of this study was primarily to follow the sporogony cycle ofPlasmodium vivax in main vector, Anopheles stephensi mysorensis andthen the effect of inhibitor carbohydrates on development of the parasite.An. stephensi were fed with the blood contains gametocyte from donormalaria patients in a restricted insectary. The development of plasmodiumwas followed by dissecting the infect mosquitoes and taking smear indifferent interval times. Subsequently, different groups of Anopheles wereseparately fed with infected blood contain inhibitor carbohydrates asfollow; N-acetyle-glucoseamin, N-acetyle- galactoseamin, arabinose,fucose, manose, lactose, galactose and glucose.Exfellagelation was occurred at 5 minutes after blood meal and thenookinet was observed at 20 hours, while oocysts and sporozoites appeasedin day’s 8 th to 12 th . The results indicate that An. stephensi strain mysorensishas can extremely transfer Pl. vivax. The parasite development wascompleted in the mosquitoes, which had been fed with N-acetylglucoseamine,arabinose, fucose , galactose and glucose, in contrast,lactose, mannose and N-acetyl-galactosamine interrupted the life cycle ofthe parasite and do not allow sprorosoite to take place into salivary glands.It seems that several carbohydrate binding protein involved in invasion anddevelopment of the parasites in mosquito. However, the results of thisstudy can be used as basic information for developing vaccine againstmalaria transmission.Key Words: malaria, sporogony cycle, Anopheles stephensi, sporozoite,carbohydra18