Abai, MR

6th International Congress of DipterologyPhysiological studies of four forensically important fliesfound in different seasons in JapanMoribayashi A., H. Kurahashi, T. Hayashi, Y. Tsuda & M. KobayashiDepartment of Medical Entomology, National Institute of Infectious Diseases. Toyama 1-23-1, Shinjuku-ku, Tokyo, 162-8640, JAPANFlies that develop in different seasons have different physiologicalcharacters and life styles. We studied a physiology of flesh flies,Boettcherisca peregrina and Parasarcophaga similis, that breed late springto early autumn and of blow fly, Aldrichina grahami and Calliphoranigribarbis, that breed late autumn to early spring in Japan. Larvae of theseflies feed on dead animals and dead bodies, so that they are forensicallyimportant. Flesh fly larviposit first inster larvae, while blow flies oviposit.Larvae of the flesh fly enter pupal diapause under a short day condition at20°C but do not enter diapause under a long day condition. Larvae destinednon-diapause develop adults in 4 weeks at about 20°C. Flesh fly femalescan larviposit in 10 days at 25°C, and it takes 5 days from first inster larvaeto mature larvae. Mature larvae are inhibited to become pupae by contactwith a little water (water immersion treatment). The treated mature larvaesurvived 20 days at 25°C and 30 days at 20°C, respectively. Percentages oftriacylglycerol (TG) and arachidonic acid of lipid extracted from thesurvived larvae especially decreased by water treatment. Adult blow flies,A. grahami and C. nigribarbis aestivate in highlands or northern areas insummer, and appear in lowlands during autumn to winter, which is a shortday condition, and lay eggs. In the experimental condition at 20°C under ashort day condition, A. grahami can lay eggs 6 or 7 day after eclosion butC. nigribarbis takes about 21 days. High peaks of ecdysteroid hormone foregg development of A. grahami and C. nigribarbis were found only infemales grown under a short day condition. After adult emergence,percentage of palmitoleic acid increased gradually and the acid finallybecame more than 40%.Key Words: Boettcherisca peregrina, Parasarcophaga similis, Aldrichinagrahami, Calliphora nigribarbis, ecdysteroid hormone168