Abai, MR

6th International Congress of DipterologyKey Words: Liriomyza sativae, Liriomyza trifolii, Liriomyza bryoniae,seasonal prevalence of occurrence, species composition∗∗∗∗∗∗∗∗∗∗Solving basal phylogeny and some taxonomic problemswithin family Ceratopogonidae (Diptera) using 16S rDNAsequencesTóthová, A. (1), J. Knoz, (2), J. Bryja (1, 3) & J. Vaňhara (1)(1) Institute of Botany and Zoology, Faculty of Science, Masaryk University, Kotlárská 2,611 37 Brno, Czech Republic(2) Institute of Applied Biology, Faculty of Science, Masaryk University, Kotlárská 2,611 37 Brno, Czech Republic(3) Institute of Vertebrate Biology, Academy of Sciences CR, 675 02 Studenec 122,Czech RepublicThe family Ceratopogonidae is a diverse and interesting family of bitingflies containing vectors of viruses, protoza and nematodes as well.Sequences from the mitochondrial 16S rDNA gene were analysed for 16species of the family Ceratopogonidae representing 6 genera of 3subfamilies. Phylogenetic reconstruction by NJ, MP and MP methods gavea strong support to the monophyly and the recent systematics of the family.The analysis of the 16S rDNA sequences supported the separation of thefamily to the subfamily and tribe level. We documented also the ability of16S rRNA gene sequences to distinguish species within a genus. Obtainedresults in general confirmed the intrageneric classification and helped toresolve some taxonomical problems, i.e. supported the validity of speciesDasyhelea saxicola, D. versicolor and D. septuosa, which are very variablein their morphological characters and difficult to identify on the basis oftheir morphology. The only phylogenetic study based on molecular dataanalysed sequences of the cox2 gene and supported the monophyly of thefamily as well as the recent classification based on morphological271