Abai, MR

6th International Congress of DipterologyMorphological and Molecular Identification of ForensicallyImportant Sarcophagidae (Diptera) in TaiwanTeng, J.-L. (1), H. Kurahashi (2) & C.-H. Yang (1)(1) Department of Forensic Sciences, Central Police University56 Shu-Jen Road,Kueishan, Taoyuan, Taiwan 33334(2) Department of Medical Entomology, National Institute of Infectious Diseases,Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, JapanBy the analysis of the species found on a corpse and the record of theambient temperature, investigators are able to estimate the date of death.Here we report the forensically important Sarcophagid flies in Taiwan.Forty-seven samples of Sarcophagid flies were collected from differentarea of Taiwan. The species identifications were based on morphology andDNA analysis of the cytochrome b oxidase subunit I mitochondrial gene.Six species, Parasarcophaga crassipalpis, Parasarcophaga misera,Parasarcophaga dux, Parasarcophaga ruficornis, Boettcherisca pergrina,Seniorwhitea princeps were initially identified by morphology. Then theDNA analysis results showed that the COI sequence variation within theintraspecies of Parasarcophaga dux is 1-2%. We suspected that there werethree groups within the populations of Parasarcophaga dux in Taiwan area.Re-examine the specimens of dux found that they are morphologically verysimilar to each other, but there are somewhat difference among these threegroups. Each group is not region specific, but can be collected fromdifferent region.. We also found that the DNA sequence variation betweenthe individual of Boettcherisca pergrina ranging from 1 to 3%. The reexamineof the morphology difference is performing. The variation in theParasarcophaga dux and that of Boettcherisca pergrina will be discussedin the paper.Key Wordsforensic entomology, Sarcophagidae, mitochondrial DNA,intraspecies∗∗∗∗∗∗∗∗∗∗263