Abai, MR

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6th International Congress of Dipterologycharacters (Beckenbach & Borkent 2003). However, cox2 gene was nottested for its usefullnes on interspecific level.First, we used the mitochondrial 16S rRNA gene sequences to analyse amolecular phylogeny of the family and compared the generic relationshipswith the established phylogeny of this group based on morphology andfossil records. Second, we tested the usefullnes of 16S gene marker on thegenus and species level and by using these sequences we tried to revise thevalidity of problematic taxa from the genus Dasyhelea.The study was financially supported by the PhD Research Fellowship ofMinistry of Education GA ČR 524/05/H536 and grant MSM No.0021622416 of the Masaryk University.Key Words: Ceratopogonidae, 16S, phylogeny, Diptera, Dasyhelea∗∗∗∗∗∗∗∗∗∗272
6th International Congress of DipterologyPredation by Crossopalpus hirsutipes Collin (Diptera:Empididae) on adult Liriomyza sativae Blanchard (Diptera:Agromyzidae)Tran, AN T.T. (1), A. Andersen (2) & T.C. Bui (1)(1) Department of Plant Protection, Nonglam University, Hochiminh City, Thuduc,Vietnam.(2) Department of Entomology and Nematology, The Norwegian Crop Research Institute,Høgskoleveien 7, N-1432 Aas, Norway and Norwegian University of Life Sciences,Department of Plant and Environmental sciences, Høgskoleveien 7, N-1432 Aas,Norway.The fly species Crossopalpus hirsutipes Collin (Diptera: Empididae) wasfound to be an important predator of adults of the leafminer Liriomyzasativae Blanchard (Diptera: Agromyzidae) infesting vegetables inHochiminh city, Vietnam. We successfully reared the predator in thelaboratory for 15 generations by using the earthworm Perionyx excavatusPerrier (Oligochaeta: Lumbricidae) as food for the larvae, and adult L.sativae flies, adult Drosophila melanogaster (Diptera: Drosophilidae) fliesand nymphs, and adults of the leafhopper Empoasca flavescens Fabricius(Homoptera: Cicadellidae) as prey for the adults. All cultures were heldand all experiments were done at 27°C, 12L:12D photoperiod and 65% RH.The average time from oviposition to adult emergence of C. hirsutipes was22 days. The mean adult longevity was 41 days for females and 36 days formales, and the mean lifetime fecundity was 23.5 eggs per female. Singleadults preyed on 14-20 (mean: 17.3) L. sativae adults per day, and malesand females ate the same amount. When offered four different species asprey in no-choice predation experiments, C. hirsutipes fed more frequentlyon adult L. sativae than on adult D. melanogaster and nymphs and adultsof Empoasca sp., and least frequently on adult D. radicum (Diptera:Anthomyiidae)Key Words: earthworms, laboratory rearing, leafminers, life history273
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Abai, MR

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