Abai, MR

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6th International Congress of DipterologySome photosynthetic pigment-protein complexes are lifetimedeficient in two cecidomyiid galls derived from Machilusthunbergii leaf.Yang, C.-M. (1)*, M.-M. Yang (2), J.-M. Hsu (3) & W.-N. Jane (4)(1) Research Center for Biodiversity, Academia Sinica, Nankang, Taipei, Taiwan 115,ROC(2) Department of Entomology, National Chung-Hsing University, Taichung, Taiwan 250,ROC(3) Institute of Plant Science and Microbiology, Academia Sinica, Nankang, Taipei,Taiwan 115, ROC*Corresponding author: cmyang@gate.sinic.edu.twTwo cecidomyiid galls at young, greening and mature developmentalstages, derived from Machilus thunbergii Sieb & Zucc leaves werecollected from the Yang-Ming Shan National Park at northern Taiwan.Their biochemical composition of photosynthetic pigment-proteincomplexes located in thylakoid membranes were analyzed by using of theThornber and MARS electrophoretic fractionation systems. Both gallswere found to be totally deficient in pigment-protein complexes CPI, andA1, AB1, and AB2, respectively, through the whole period of gallformation. Immunoblotting of antibody against LHCIIb apoproteinconfirmed this lifetime deficiency, which never recovered under anycondition. Electron microscopy demonstrated that even at the earlydevelopmental stage the galls still have thylakoid morphology as normal asleaf. However, it is unknown how the cecidomyiid insects cause thedeficiency of some pigment-protein complexes in gall chloroplasts andwhether the unique phenomenon is ubiquitous or just a special case.Key Words: cecidomyiid gall, LHCIIb, Machilus thunbergii, pigmentproteincomplexes∗∗∗∗∗∗∗∗∗∗318
6th International Congress of DipterologyThe expression of the cecropin genes in Armigeres subalbatusYang, C. Y. (1), W.T. Liu (1), C.C. Chen (2), W.C. Tu (3) & K.H. Lu (3)(1) Medical Insects Laboratory, Graduate School of Entomology Studies, National ChungHsing University, South District, Taichung City 40227, Taiwan (R.O.C.)(2) Institute of Parasitology, National Yang-Ming University, Beitou District, Taipei City112, Taiwan (R.O.C.)(3) Institute of Entomology, National Chung Hsing University, South District, TaichungCity 40227, Taiwan (R.O.C.)Five cecropin genes, AsCec 1, AsCec 2, AsCec 4, AsCec 6, AsCec 7, wereexpressed in the adult mosquito, Armigeres subalbatus, by inoculatingheat-killed Escherichia coli. Most expressed genes were inducible exceptAsCec 6. However, an interesting phenomenon observed in AsCec 6 wasthe constitutive expression in both adult and larval stages, which was notaffected by the microbial challenge as that of Staphylococcuc aureus,Nomuraea rileyi, and E. coli. A novel gene-expressed profile was alsoobserved in AsCec 6 that the expression of AsCec 6 declined 1 hr afterpupation and then disappeared till 48 hr, but expressed again at 72 hr afterpupation. During the early period of pupae, AsCec 6 was inducible andpersistently expressed until 18 hours with E. coli treatment, then graduallydecreased to lowest at 36 hr, and finally rise again from 48 hr tillemergence. Furthermore, the gene function of AsCec 6 was verified byusing the RNAi technique. Silencing of AsCec 6 resulted in the failure ofemergence or morphological abnormal of adult legs. The results reveal thatAsCec 6 may serve as peptide antibiotics as well as play an important rolein the emergence of Ar. subalbatus.Key Words: cecropin, Armigeres subalbatus∗∗∗∗∗∗∗∗∗∗319
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Abai, MR

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