Abai, MR

6th International Congress of DipterologyA Supertree for the stiletto flies (Diptera: Therevidae) usingconstraint trees and the parsimony ratchet to overcome lowtaxon overlapLambkin, C.L. (1), J.W.H. Trueman (2), D.K. Yeates (1), K.C. Holston (3), D.W. Webb(4), M. Hauser (5), M.A. Metz (6), H.N. Hill (7), J.H. Skevington (8), L. Yang (9), M.E.Irwin (10), & B.M. Wiegmann (11)(1) CSIRO Entomology, Canberra ACT 2601 AUSTRALIA(2) School of Botany and Zoology, Australian National University, Canberra ACT 0200,AUSTRALIA(3) Swedish Museum of Natural History, S-104 05 Stockholm, SWEDEN(4) Center for Biodiversity, Illinois Natural History Survey Champaign, IL 61820, USA(5) California Department of Food & Agriculture, Sacramento, CA 95832-1448, USA(6) NMNH, Smithsonian Institution, Washington, DC 20560-0168, USA(7) U.S. Environmental Protection Agency, Washington, DC 20460, USA(8) Agriculture and Agri-Food Canada, Ottawa, ON K0A 3M0, CANADA(9) CIIT Centers for Health Research, Research Triangle Park, NC 27709, USA(10) Dept. Natural Resources and Environmental Science, University of Illinois, Urbana,IL 61801, USA(11) Department of Entomology, North Carolina State University, Raleigh, NC 27695,USAWe explore the use of the three most common approaches to calculatingglobally inclusive phylogenies from smaller, more exclusive analyses;supertree methods, ‘supermatrix’ methods, and total evidence. Supermatrixand total evidence approaches are often unsuitable for use in species-richclades such as insects because these methods require significant overlap inboth terminal taxa and data classes. Supertree methods require no overlapin data class between source analyses, performing well even withincomplete taxon overlap; hence they are suitable for use in species-richclades. We describe a method for handling low taxon overlap in supertreeanalyses. A therevid supertree was produced using matrix representationwith parsimony (MRP) analysis in combination with the parsimony ratchetand constraint tree techniques, from 24 phylogenetic studies containing atotal of 362 terminal taxa despite only 34% of the terminal taxa found inmore than one source tree. The resulting supertree is a completephylogenetic hypothesis for Therevidae that incorporates extensive150