kasetsart journal natural science

insignificant differences on specific CAT activity
observed under all conditions studied. A relatively
stable CAT production of C. sithepensis sp. nov.
was concurrent to those appeared in C. boidinii
cultures (Volfova et al., 1992).
OD 610
Methanol (%v/v)
100
10
1
0
2.00
1.50
1.00
0.50
0.00
0 12 24 36 48 60
Times (h)
Kasetsart J. (Nat. Sci.) 40(1) 129
A
B
Figure 6 summarizes the results of timecourse
determination for growth and enzymatic
profiles of P. siamensis sp. nov. Stationary phase
growth of P. siamensis sp. nov. under low methanol
supplement (0.75%) at its optimum temperature
Specific activity (u/mg protein)
8
6
4
2
0
4000
3000
2000
1000
0
2.5
2.0
1.5
1.0
0.5
0.0
0.5
0.4
0.3
0.2
0.1
0.0
0 12 24 36 48 60
Times (h)
Figure 6 Growth (A), methanol residue (B) and specific activities of alcohol oxidase (C), catalase (D),
glutathione-dependent formaldehyde dehydrogenase (E) and formate dehydrogenase (F) of
P. siamensis sp. nov. cultivated at various growth conditions. 37 °C and 0.75% (v/v) methanol,
(▲); 37 °C and 2% (v/v) methanol, ( ); 40 °C and 0.75% (v/v) methanol, (■); 40 °C and
2% (v/v) methanol, ( )
E
F
C
D