kasetsart journal natural science

More documents

Recommendations

Info

Kasetsart J. (Nat. Sci.) 40(1) 161 Figure 1 A) A soft-shell crab farm in Klung District, Chanthaburi Province B) A normal mud crab (Scylla serrata) C) A red sternum mud crab showing the first sign of red sternum symptom with pink sternum D) The late phase of red sternum showing dark red sternum, chelae, and joint E) and F) Comparing the inner organs of normal (E) and red sternum mud crab (F) Showing deterioration of the integument, gills, and hepatopancreas G) Collecting haemolymph from the base of pereiopods H) Normal transparent haemolymph and milky unclotted haemolymph of a red sternum mud crab
162 warm, relatively shallow and high salinity water (Burnett, 1992). The ventral sides of their limbs were opaque, bright white color, rather than the normal translucent whitish-gray color. Dorsally, the carapace was slightly pinkish as opposed to the normal orange-tan color. In the case of PCD and BCD, Hematodinium consumes oxygen from the crab’s blood and tissues which cause it to become weak and lethargic (Meyers et al., 1996). The haemolymph of PCD and BCD infected crab is milky white in color rather than the normal translucent light gray. When cooked, the crabmeat had a chalky texture and a bitter aspirin-like flavor (Meyer et al., 1987). Stentiford et al. (2001) reported on the composition of Hematodiniuminfected in the Norway lobster (Nephrops norvegicus) tissues and suggested that disruptions in the normal carbohydrate and amino acid profiles of these tissues might cause the bitter taste of the meat. However, PCD and BCD had no impact on people who ate infected crab, but the parasites could be detected under a microscope (Meyers et al., 1996). Once crab got infected, Hematodinium grew rapidly inside the crab (up to 10 6 parasites/ml of blood) over the course of 3 to 6 weeks, the crab’s blood changed to a milkywhite color and lost its clotting ability (Stentiford et al., 2002). Although the outer appearances of red sternum, PCD and BCD were quite similar, it could not be conclude that the causes of these symptoms would be the same. Further investigation to clarify its cause needs to be done. SDS-PAGE The blood protein of red sternum mud crab was compared to that of normal mud crab using SDS-PAGE analysis. There were several intense protein bands found in the ranges of 66- 97 kDa in the normal crab haemolymph, while the haemolymph of red sternum mud crab had no band at all in this range (Figure 2). Since the molecular weight of oxyhemocyanin is 75 kDa and this substance was the main component of blood Kasetsart J. (Nat. Sci.) 40(1) Figure 2 Lane A) intense protein band of 75 kDa in normal haemolymph Lane B) disappearance of 75 kDa band in haemolymph of late phase red sternum symptom Lane C) molecular markers Lane D) less intense band of 75 kDa in haemolymph of mud crab having first sign of red sternum symptom. protein found in white shrimp (Penaeus vannamei) (Figueroa-Soto and Barca, 1997), the distinct band of 66-97 kDa suggested the existence of oxyhemocyanin in normal mud crab haemolymph and the gradual disappearance of this band (Figure 2) could be the clear marker of changing in blood component of this symptom as shown by the unclotted and milky-like substances. To confirm this result, spectroscopic analysis was used to reveal the different spectra of haemolymph of red sternum and normal mud crab. In Figure 3, the spectrum of normal crab haemolymph showed the maximum absorbance at 340 nm representing the absorbance of oxyhemocyanin (Terwilliger et al., 1999) but it was absent in the red sternum crab haemolymph. Haemocyanin (Hc) is a copper-
Page 1 and 2:
January - March 2006 Volume 40 Numb
Page 3 and 4:
KASETSART JOURNAL NATURAL SCIENCE T
Page 5 and 6:
In sacco Degradation Characteristic
Page 7 and 8:
2 and management practices a sorghu
Page 9 and 10:
4 the greater plant height was obta
Page 11 and 12:
6 at Wolenchity (Figure 3) that led
Page 13 and 14:
8 Stover and aboveground biomass Co
Page 15 and 16:
10 Radford, B.J., A.J. Dry, L.N. Ro
Page 17 and 18:
12 At present, new cultivars with h
Page 19 and 20:
14 2000), grapevine (Lavee, 2000),
Page 21 and 22:
16 repeatability of FW, FLT, FLW, S
Page 23 and 24:
18 (Table 5) indicating that select
Page 25 and 26:
Kasetsart J. (Nat. Sci.) 40 : 20 -
Page 27 and 28:
22 measured to define fruit shape i
Page 29 and 30:
24 for higher fruit number and yiel
Page 31 and 32:
Page 33 and 34:
28 (Table 2). The numbers of flower
Page 35 and 36:
30 to the color of fruit and seed c
Page 37 and 38:
32 1999. Carrots and related vegeta
Page 39 and 40:
34 MATERIALS AND METHODS Laboratory
Page 41 and 42:
36 DISCUSSION Extensive studies hav
Page 43 and 44:
38 from Gossypium hirsutum. J. Inse
Page 45 and 46:
40 The natural resistance of plants
Page 47 and 48:
42 4. Data collection and statistic
Page 49 and 50:
44 was not significantly different
Page 51 and 52:
46 showed the highest yield compare
Page 53 and 54:
48 Kessmann, H., T. Staub, C. Hofma
Page 55 and 56:
50 There are approximately 60 comme
Page 57 and 58:
52 in PBST containing 2% ovalbumin
Page 59 and 60:
54 Kasetsart J. (Nat. Sci.) 40(1) T
Page 61 and 62:
56 Virus-free orchid plants show fa
Page 63 and 64:
Page 65 and 66:
60 feed source was based on purchas
Page 67 and 68:
62 intake of DM, CP, ether extract
Page 69 and 70:
64 relatively slow and short. The p
Page 71 and 72:
66 week 4. Milk composition was not
Page 73 and 74:
68 Agricultural Experiment Station.
Page 75 and 76:
70 et al., 2002). In contrast to ma
Page 77 and 78:
72 level and showed marked polymorp
Page 79 and 80:
Page 81 and 82:
76 loam (Eutric Fluvisol) soil and
Page 83 and 84:
78 Eugenia caryophyllata, Echinops
Page 85 and 86:
80 CONCLUSION In conclusion, out of
Page 87 and 88:
82 of chromic acid titration method
Page 89 and 90:
84 initials at the time of pinning
Page 91 and 92:
86 vessels a point where the number
Page 93 and 94:
88 Kasetsart J. (Nat. Sci.) 40(1) T
Page 95 and 96:
90 CONCLUSIONS In the investigation
Page 97 and 98:
92 1994; Strand et al., 1997). Now
Page 99 and 100:
94 The clones were grown overnight
Page 101 and 102:
96 isozymes. The copy number for nu
Page 103 and 104:
98 Helianthus annuus. Theor. Appl.
Page 105 and 106:
100 MATERIALS AND METHODS Plant mat
Page 107 and 108:
102 The amount of Na + (%) 6 5 4 3
Page 109 and 110:
104 NGE, 7-16 folds). Finally, all
Page 111 and 112:
106 Anal. Biochem. 162: 156-159. Cr
Page 113 and 114:
108 grown for academic purpose at N
Page 115 and 116: 110 Cluster A was further separated
Page 117 and 118: 112 Table 2 Similarity index of 19
Page 119 and 120: 114 subjected to UPGMA and resultin
Page 121 and 122: 116 Tabel 3 The similarity index of
Page 123 and 124: 118 formation as seen from phylogen
Page 125 and 126: 120 Genet. 101: 860-864. Lerceteau,
Page 127 and 128: 122 INTRODUCTION Methylotrophic yea
Page 129 and 130: 124 RESULTS AND DISCUSSION Screenin
Page 131 and 132: 126 optimum temperature, insignific
Page 133 and 134: 128 medium of C. boidinii (Volfova
Page 135 and 136: 130 was observed soon after 24h cul
Page 137 and 138: 132 Kasetsart J. (Nat. Sci.) 40(1)
Page 139 and 140: 134 ACKNOWLEDGEMENTS This work was
Page 141 and 142: Kasetsart J. (Nat. Sci.) 40 : 136 -
Page 143 and 144: 138 Determination of enzyme activit
Page 145 and 146: 140 Table 1 Optimum and stability o
Page 147 and 148: 142 for example recombinant E. coli
Page 149 and 150: 144 were studied for β-1,3-1,4-glu
Page 151 and 152: 146 ACKNOWLEDGEMENT This work was s
Page 155 and 156: 150 vulcanization times were calcul
Page 157 and 158: 152 RESULTS AND DISCUSSION Mechanic
Page 159 and 160: 154 Kasetsart J. (Nat. Sci.) 40(1)
Page 161 and 162: 156 blend with 0.5 phr Irganox. How
Page 165: 160 (AA-680 ShiMADZU, Atomic Absorp
Page 169 and 170: 164 cycle in crustacean (Chen et al
Page 171 and 172: 166 Salaenoi, J. 2004. Changes of e
Page 173 and 174: 168 MATERIALS AND METHODS This rese
Page 175 and 176: 170 DISCUSSION The average total am
Page 179 and 180: 174 repacked and scanned three time
Page 181 and 182: 176 found the most in the shrimp fe
Page 183 and 184: 178 PLS model The comparison betwee
Page 185 and 186: 180 brix value and dry matter of ma
Page 187 and 188: 182 approach to increase immunity t
Page 189 and 190: 184 higher (P
Page 191 and 192: 186 levels were significantly eleva
Page 195 and 196: 190 currents. Zone 4: River outlet
Page 197 and 198: 192 standing waters, thus the occur
Page 199 and 200: 194 and moving to 59%TL in juvenile
Page 203 and 204: 198 thin membrane (Figure 1A, B). T
Page 205 and 206: 200 were clearly seen. The presence
Page 207 and 208: 202 could change to female at 6-12
Page 211 and 212: 206 water until the water became cl
Page 213 and 214: 208 reported by Thu and Preston (19
Page 215 and 216: 210 cavalcade hay. The crude protei
Page 217 and 218:
212 CONCLUSIONS Ruminal disappeared
Page 219 and 220:
214 ∅rskov, E.R. and I. McDonald.
Page 221 and 222:
216 is constrained mainly due to fa
Page 223 and 224:
218 SNF and fat composition in raw
Page 225 and 226:
220 in terms of both manual samplin
Page 227 and 228:
222 Harding, F. 1995. Compositional
Page 229 and 230:
224 proteins described by Barbut an
Page 231 and 232:
226 starch/flour, shaken vigorously
Page 233 and 234:
228 % Stress relaxation 54 49 44 39
Page 235 and 236:
230 Kasetsart J. (Nat. Sci.) 40(1)
Page 237 and 238:
Page 239 and 240:
234 categories including puffed sna
Page 241 and 242:
236 Cohesiveness of mass Crispness
Page 243 and 244:
238 Cohesiveness of mass Sweetness
Page 245 and 246:
Page 247 and 248:
242 3. Physicochemical properties 3
Page 249 and 250:
Page 251 and 252:
246 ACKNOWLEDGEMENTS This research
Page 253 and 254:
248 are now becoming more and more
Page 255 and 256:
250 downstream control. Lam Chae re
Page 257 and 258:
Page 259 and 260:
254 Inflow (mcm.) Inflow (mcm.) Inf
Page 261 and 262:
256 were above 0.70. The testing re
Page 263 and 264:
258 1999-2000 could be used for tra
Page 265 and 266:
Page 267 and 268:
262 we assume that r ≥ 2 and d r
Page 269 and 270:
Page 271 and 272:
266 Figure 1 Prototype domains. num
Page 273 and 274:
268 DISCUSSION Consequences indicat
Page 275 and 276:
Page 277 and 278:
272 George, B. K. 1997. The GIS Boo
Page 279 and 280:
discussed; and appropriateness of t
Page 281 and 282:
LITERATURE CITED FORMAT Manuscripts
Page 283 and 284:
PRE-SUBMISSION CHECKLIST (see “In
Page 285 and 286:
Send application materials to Payme
Page 287:
Text and Journal Publication Co., L
show all

kasetsart journal natural science

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?