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Kasetsart J. (Nat. Sci.) 40(1) 133 S-AOD1 NPWVYLPGVYPRNMRLDSKTATFYTARPSPHLNGRRAIVPCANILGGGSSINFLMYTRAS PmMOD1 NPWVYLPGVYPRNMRLDSKTATFYSSRPSPHLNGRRAIVPCANILGGGSSINFLMYTRAF FS-AOD NPWVYLPGVYPRNMRLDSKTATFYTSRPSKALNGRRAIVPCANILGGGSSINFLMYTRAS HpMOX NPWVYLPGVYPRNMRLDSKTATFYSSRPSKALNGRRAIVPCANILGGGSSINFLMYTRAS S-AOD2 NPWVYLPGVYPRNMRLDSKTATFYNSRPSPHLNGRRAIVPCANILGGGSSINFMMYTR-- PmMOD2 NPWVYLPGVYPRNMRLDSKTATFYSSRPSPHLNGRRAIVPCANILGGGSSINFMMYTRGF CbAOD1 NPWVYLPGIYPRNMRLDSKTATFYNSRPSKHLNGRRAIVPQANILGGGSSINFMMYTRAS PpAOX1 NPWVYLPGIYPRNMKLDSKTASFYTSNPSPHLNGRRAIVPCANVLGGGSSINFMMYTR-- PpAOX2 NPWVYLPGIYPRNMKLDSKTASFYTSNPSPHLNGRRAIVPCANILGGGSSINFMMYTRGF ********:*****:******:**.:.** ********* **:*********:**** S-AOD1 --ASDYDDWEMEGWKTDDLLPLMKKLETYQRPCNNRDLHGFDGPIKVSFGNYTYPHCQDF PmMOD1 TSASDYDDWESEGWTTDELLPLMKKIETYQRPCNNRELHGFDGPIKVSFGNYTYPNGQDF FS-AOD --ASDYDDWESEGWTTDELLPLIKKIETYQRPSNNRDLHGFDGPIKVSFGNYTYPTCQDF HpMOX --ASDYDDWESEGWSTDELLPLIKKIETYQRPCNNRDLHGFDGPIKVSFGNYTYPTCQDF S-AOD2 ASASDYDDWETEGWTTDDLLPLMKKLETYQRPSNNRELHGFDGPIKVSFGNYTYPNCQDF PmMOD2 TSASDYDDWESEGWTTDELLPLMKRLETYQRPCNNPDLHGFDGPIKVSFGNYTYPNCQDF CbAOD1 --ASDYDDWESEGWTTDELLPLMKKFETYQRPCNNRDVHGFDGPIKVSFGNYTYPQCQDF PpAOX1 GSASDYDDFQAEGWKTKDLLPLMKKTETYQRACNNPDIHGFEGPIKVSLGNYTYPVCQDF PpAOX2 TSASDYDDFEAEGWKTKDLLPLMKKTETYQRACNNPEIHGFEGPIKVSFGNYTYPVCQDF ******:: ***.*.:****:*: *****..** ::***:******:****** *** S-AOD1 L--RAAESQGIPFVDDAEDLTTAHGAEHWLKWINRDLGRRSDSAHAYIHPTMRNKQNLYL PmMOD1 FTIRAAESQGIPFVDDAEDLKCSHGAEHWLKWINRDLGRRSDSAHAYIHPTMRNKQNLFL FS-AOD LR--AAESQGIPVVDDLEDFKTSHGAEHWLKWINRDLGRRSDSAHAYIHPTMRNKQSLFL HpMOX LR--AAESQGIPVVDDLEDFKTSHGAEHWLKWINRDLGRRSDSAHAYVHPTMRNKQSLFL S-AOD2 L--RAAESQGIPFVDDAEDLTTSHAAEHWLKWINRDLGRRSDAAHAYIHPTMRNKQNLYL PmMOD2 FTLRAAESQGIPFVDDAEDLKTSHASQHWLKWINRDLGRRSDAAHAYIHPTMRNKSNLYL CbAOD1 LR--ACETQGIPYVDDLEDLKTSHGAEQWLKWINRDFGRRSDTAHAFIHSTMRNKENLFL PpAOX1 LRASES--QGIPYVDDLEDLVTAHGAEHWLKWINRDTGRRSDSAHAFVHSTMRNHDNLYL PpAOX2 FTLRATESQGIPYVDDLEDLVTAHGAEHWLKWINRDTGRRSDSAHAFVHSTMRNHDNLYL : **** *** **: :*.:::******** *****:***::*.****:..*:* S-AOD1 ITST--KCDKVIIENGTAVAVKTVPMKPTGS-PKTAVARTYRARKQIVVSCGTISSPLVL PmMOD1 IFTTSTKCEKIIIENGVATGIKTVPMKPTGS-PKTQVARTFKARKQIIVSCGTISSPLVL FS-AOD ITST--KCDKVIIEDGKAVAVKTVPMKPLN--PKKPVSRTFRARKQIVISCGTISSPLVL HpMOX ITST--KCDKVIIEDGKAVAVRTVPMKPLN--PKKPVSRTFRARKQIVISCGTISSPLVL S-AOD2 ITST--KADKIIIEDGVAVAVKTVPTKPVGGASAKSHGRTYRAKRQIIISSGTISSPQIL PmMOD2 IFTTSTKADKVIIEDGVAAGIQVVPSKPLN--PEKPAAKIYKARKQIILSCGTISTPLVL CbAOD1 MTNT--KVDKVIIEDGRAVAVRTVPSKPIGD---SKVSRTFKARKQIVVSCGTVSSPMVL PpAOX1 ICNT--KVDKIIVEDGRAAAVRTVPSKPLNP--KKPSHKIYRARKQIVLSCGTISSPLVL PpAOX2 IFTCNTKVDKIIVEDGRAAAVRTVPSKPLNA--KKPTHKVYRARTQIVLSCGTISSPLVL : . * :*:*:*:* *..::.** ** . . : ::*: **::*.**:*:* :* S-AOD1 QR--SGIGAAHKLRQVGIKPIVDLPGVGLNFQDHHCFFTPYYVKPDVPTFDDFVRGDKAV PmMOD1 QRSFTGIGSAHKLRQVGIKPVVDLPGVGMNFQDHYCFFTPYHVKPDTPSFDDFVRGDKAV FS-AOD QR--SGIGAAHHLRSVGVKPIVDLPGVGENFQDHYCFFTPYHVKPDVPTFDDFVRGDPVA HpMOX QR--SGIGAAHHLRSVGVKPIVDLPGVGENFQDHYCFFTPYYVKPDVPTFDDFVRGDPVA S-AOD2 ERS--GVGSAHKLRQAGIKPIVDLPGVGENFQDHYCYFVPYHVKPDTPSFDDFVRGDKET PmMOD2 QRSGFTIGSAHKLRQAGIKPIVDLPGVGMNFQDHYCFFTPYHVKPDTPSFDDFARGDKAV CbAOD1 QRS--GIGEPSKLRAAGVKPIVELPGVGRNFQDHFCYFVPYRIKQDSESFDAFVSGDKEA PpAOX1 QRS--GFGDPIKLRAAGVKPLVNLPGVGRNFQDHYCFFSPYRIKPQYESFDDFVRGDAEI PpAOX2 QRSGFTFGDPIKLRAAGVKPLVNLPGVGRNFQDHYCFFSPYRIKPQYESFDDFVRGDANI :* .* . :** .*:**:*:***** *****.*:* ** :* : :** *. ** S-AOD1 QKS--AFDQWYANKDGPLTTNGIEAGVKIRPTEEELATADDEFRAAYDDYFGSKPDKPLM PmMOD1 QKSAFTFDQWYANKDGPLTINGIEAGVKIRPTEEELATADDEFRAAYDDYFGNKPDKPLM FS-AOD QKS--AFDQWYSNKDGPLTTNGIEAGVKIRPTDEELATADEDFRQGYADYFENKPDKPLM HpMOX QKA--AFDQWYSNKDGPLTTNGIEAGVKIRPTEEELATADEDFRRGYAEYFENKPDKPLM S-AOD2 QTAAFN--QWYANKDGPLTTNGIEAGVKIRPTEEELSTADDDFKDGWFSYFENKPDKPLM PmMOD2 QKSAFFTDQWYANKDGPLTTNGIEAGVKIRPTAEELATADEDFQLGYASYFENKPDKPLM CbAOD1 QKS--AFDQWYATGAGPLATNGIEAGVKIRPTEAELATADKAFQQGWESYFENKPDKPLM PpAOX1 QKRLFD--QWYANGTGPLATNGIEAGVKIRPTPEELSQMDESFQEGYREYFEDKPDKPVM PpAOX2 QKKVFFTDQWYANGTGPLATNGIEAGVKIRPTPEELSQMDESFQEGYREYFEDKPDKPVM *. ***:. ***: ************ **: *. *: .: .** .*****:* S-AOD1 HYSVISG--FFGDHTKIPNGKFMTMFHFLEY PmMOD1 HYSLIFTSGFFGDHTKIPNGKYMCMFHFLEY FS-AOD HYSVISG--FFGDHTKIPNGKFMTMFHFLEY HpMOX HYSVISG--FFGDHTKIPNGKFMTMFHFLEY S-AOD2 HYSLISG--YFGDHTKIPHGKYMTMFHFLEY PmMOD2 HYSLISFTGFFGDHTKIPNGKYMTMFHFLEY CbAOD1 HYSVISG--FFGDHTRLPPGKYMTMFHFLEY PpAOX1 HYSIIAG--FFGDHTKIPPGKYMTMFHFLEY PpAOX2 HYSIIAFTGFFGDHTKIPPGKYMTMFHFLEY ***:* :*****::* **:* ******* Figure 9 Alignment of deduced amino acid sequences of partial AOD gene(s) of the methylotrophic yeast C. sithepensis sp. nov. (S-AOD1 and S-AOD2) and P. siamensis sp. nov. (FS-AOD) with that of Pichia methanolica (PmMOD1, PmMOD2), P. pastoris (PpAOX1, PpAOX2), P. angusta (HpMOX) and Candida boidinii (CbAOD1). Identical and conserved residues are shown as *
134 ACKNOWLEDGEMENTS This work was supported by the JSPS- NRCT Kasetsart University and Yamaguchi University Core University Program (Microbial Resources), Kasetsart University Research and Development Institute (KURDI) and National Research Council of Thailand (NRCT). LITERATURE CITED Bergmeyer, H.U. 1955. Zur Messung von Katalase-Aktivitaten. Biochem. Z. 327: 255- 258. Kasetsart J. (Nat. Sci.) 40(1) Figure 10 Similarity of the AOD gene(s) of the methylotrophic yeast C. sithepensis sp. nov. and P. siamensis sp. nov. The tree was constructed by UPGMA analysis. Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248- 254. Cereghino, J. L. and J. M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24: 45-66. Demain, A. L., H. J. Phaff, C. P. Kurtzman. 1998. The industrial and agricultural significance of yeasts, pp. 13-20. In C.P. Kurtzman and J. W. Fell (eds.). The Yeasts: A Taxonomic Study, 4 th ed. Elsevier, Amsterdam.
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January - March 2006 Volume 40 Numb
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KASETSART JOURNAL NATURAL SCIENCE T
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In sacco Degradation Characteristic
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2 and management practices a sorghu
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4 the greater plant height was obta
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6 at Wolenchity (Figure 3) that led
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8 Stover and aboveground biomass Co
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10 Radford, B.J., A.J. Dry, L.N. Ro
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12 At present, new cultivars with h
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14 2000), grapevine (Lavee, 2000),
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16 repeatability of FW, FLT, FLW, S
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18 (Table 5) indicating that select
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Kasetsart J. (Nat. Sci.) 40 : 20 -
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22 measured to define fruit shape i
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24 for higher fruit number and yiel
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28 (Table 2). The numbers of flower
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30 to the color of fruit and seed c
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32 1999. Carrots and related vegeta
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34 MATERIALS AND METHODS Laboratory
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36 DISCUSSION Extensive studies hav
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38 from Gossypium hirsutum. J. Inse
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40 The natural resistance of plants
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42 4. Data collection and statistic
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44 was not significantly different
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46 showed the highest yield compare
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48 Kessmann, H., T. Staub, C. Hofma
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50 There are approximately 60 comme
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52 in PBST containing 2% ovalbumin
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54 Kasetsart J. (Nat. Sci.) 40(1) T
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56 Virus-free orchid plants show fa
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60 feed source was based on purchas
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62 intake of DM, CP, ether extract
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64 relatively slow and short. The p
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66 week 4. Milk composition was not
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68 Agricultural Experiment Station.
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70 et al., 2002). In contrast to ma
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72 level and showed marked polymorp
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76 loam (Eutric Fluvisol) soil and
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78 Eugenia caryophyllata, Echinops
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80 CONCLUSION In conclusion, out of
Page 87 and 88: 82 of chromic acid titration method
Page 89 and 90: 84 initials at the time of pinning
Page 91 and 92: 86 vessels a point where the number
Page 93 and 94: 88 Kasetsart J. (Nat. Sci.) 40(1) T
Page 95 and 96: 90 CONCLUSIONS In the investigation
Page 97 and 98: 92 1994; Strand et al., 1997). Now
Page 99 and 100: 94 The clones were grown overnight
Page 101 and 102: 96 isozymes. The copy number for nu
Page 103 and 104: 98 Helianthus annuus. Theor. Appl.
Page 105 and 106: 100 MATERIALS AND METHODS Plant mat
Page 107 and 108: 102 The amount of Na + (%) 6 5 4 3
Page 109 and 110: 104 NGE, 7-16 folds). Finally, all
Page 111 and 112: 106 Anal. Biochem. 162: 156-159. Cr
Page 113 and 114: 108 grown for academic purpose at N
Page 115 and 116: 110 Cluster A was further separated
Page 117 and 118: 112 Table 2 Similarity index of 19
Page 119 and 120: 114 subjected to UPGMA and resultin
Page 121 and 122: 116 Tabel 3 The similarity index of
Page 123 and 124: 118 formation as seen from phylogen
Page 125 and 126: 120 Genet. 101: 860-864. Lerceteau,
Page 127 and 128: 122 INTRODUCTION Methylotrophic yea
Page 129 and 130: 124 RESULTS AND DISCUSSION Screenin
Page 131 and 132: 126 optimum temperature, insignific
Page 133 and 134: 128 medium of C. boidinii (Volfova
Page 135 and 136: 130 was observed soon after 24h cul
Page 137: 132 Kasetsart J. (Nat. Sci.) 40(1)
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Page 143 and 144: 138 Determination of enzyme activit
Page 145 and 146: 140 Table 1 Optimum and stability o
Page 147 and 148: 142 for example recombinant E. coli
Page 149 and 150: 144 were studied for β-1,3-1,4-glu
Page 151 and 152: 146 ACKNOWLEDGEMENT This work was s
Page 155 and 156: 150 vulcanization times were calcul
Page 157 and 158: 152 RESULTS AND DISCUSSION Mechanic
Page 159 and 160: 154 Kasetsart J. (Nat. Sci.) 40(1)
Page 161 and 162: 156 blend with 0.5 phr Irganox. How
Page 165 and 166: 160 (AA-680 ShiMADZU, Atomic Absorp
Page 167 and 168: 162 warm, relatively shallow and hi
Page 169 and 170: 164 cycle in crustacean (Chen et al
Page 171 and 172: 166 Salaenoi, J. 2004. Changes of e
Page 173 and 174: 168 MATERIALS AND METHODS This rese
Page 175 and 176: 170 DISCUSSION The average total am
Page 179 and 180: 174 repacked and scanned three time
Page 181 and 182: 176 found the most in the shrimp fe
Page 183 and 184: 178 PLS model The comparison betwee
Page 185 and 186: 180 brix value and dry matter of ma
Page 187 and 188: 182 approach to increase immunity t
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184 higher (P
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186 levels were significantly eleva
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190 currents. Zone 4: River outlet
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192 standing waters, thus the occur
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194 and moving to 59%TL in juvenile
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198 thin membrane (Figure 1A, B). T
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200 were clearly seen. The presence
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202 could change to female at 6-12
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206 water until the water became cl
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208 reported by Thu and Preston (19
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210 cavalcade hay. The crude protei
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212 CONCLUSIONS Ruminal disappeared
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214 ∅rskov, E.R. and I. McDonald.
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216 is constrained mainly due to fa
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218 SNF and fat composition in raw
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220 in terms of both manual samplin
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222 Harding, F. 1995. Compositional
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224 proteins described by Barbut an
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226 starch/flour, shaken vigorously
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228 % Stress relaxation 54 49 44 39
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230 Kasetsart J. (Nat. Sci.) 40(1)
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234 categories including puffed sna
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236 Cohesiveness of mass Crispness
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238 Cohesiveness of mass Sweetness
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242 3. Physicochemical properties 3
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246 ACKNOWLEDGEMENTS This research
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248 are now becoming more and more
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250 downstream control. Lam Chae re
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254 Inflow (mcm.) Inflow (mcm.) Inf
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256 were above 0.70. The testing re
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258 1999-2000 could be used for tra
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262 we assume that r ≥ 2 and d r
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266 Figure 1 Prototype domains. num
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268 DISCUSSION Consequences indicat
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272 George, B. K. 1997. The GIS Boo
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discussed; and appropriateness of t
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LITERATURE CITED FORMAT Manuscripts
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