immunology of infectious and parasitic diseases - XXXVII Congress ...

DETECTION OF GITR ON PATIENT CERVIX INFECTED BY HUMAN
PAPILLOMAVIRUS (HPV)
CACILDA TEZELLI JUNQUEIRA PADOVANI (1) ; CAMILA MARETI BONIN (1) ;
INÊS APARECIDA TOZETTI (1) ; ALDA MARIA TEIXEIRA FERREIRA (1) ;
CARLOS EURICO DOS SANTOS FERNANDES (1) ; THIAGO THEODORO
MARTINS PRATA (1) ; SIMONE DOMINGUES SALES (1) ; ANA PAULA
MACHADO (1) ; FLÁVIA GATTO DE ALMEIDA (1) ; IZAIAS PEREIRA DA
COSTA (1) .
(1) Universidade Federal de Mato Grosso do Sul.
INTRODUCTION: The Human papillomavirus (HPV), in the anogenital tract,
infects basal and parabasal cells of squamous epithelium. The progression of
the infection has also been associated with the participation of cells with
regulatory properties. Evidence shows that GITR (glucocorticoid-induced tumor
necrosis factor receptor) is the second most important surface molecule for the
characterization of these cells. This marker, TNFRS superfamily member, is
predominantly expressed on CD25 + CD4 + Treg cells and has a strong
engagement with these phenotypic and functional cells. So, playing an
important role on regulating the immune response. Thus, this study aimed to
detect the presence of GITR and CD25 in the patient cervix stroma with and
without pathological changes, HPV infected or not. Therefore, to associate the
outcomes found with histological findings and viral load, to a better
understanding of the immune response in infected microenvironment.
METHODS AND RESULTS: Were used to this study forty-nine cervical
samples, included in paraffin, obtained from patients that were submitted to
HPV DNA detection by Hybrid Capture (Digene TM ), to the histopathology and
to immunohistochemistry analyzes (IHC) of simple marking with antigenic
recovery by moist heat and LSAB+Sys HRP (DAKO ® ) detection system for the
presence of CD4. These samples were analyzed by immunohistochemistry for
the detection of GITR (R & D systems ®, goat IgG / cod. AF689) and CD25
(Invitrogen ®, clone 143-13/cod.AHS2512) in lymphocytes. This study was
approved by CEP/UFMS (nº 975/2007). It was observed that the GITR
expression raised with the viral load (p = 0.024) and that the frequency of this
marker, in large quantities, in the HPV-positive samples (76.9%) was higher
than three times as compared to the negative samples (23.1%). There was
statistical significance between the presence of GITR and histopathological
findings (p = 0.034), with a GITR predominance, in large quantities, in highgrade
lesion samples and carcinoma (53.95%). The results showed a statistical