immunology of infectious and parasitic diseases - XXXVII Congress ...

CYTOKINE PROFILE IN HUMAN SCHISTOSOMIASIS AND ITS
RELATIONSHIP WITH FIBROSIS AND TREATMENT
GABRIELA DA SILVEIRA E NUNES 1,2 ; MÔNICA MARIA DE ALMEIDA 2 ;
AMANDA CARDOSO DE OLIVEIRA SILVEIRA 3 ; GIOVANNI GAZZINELLI 4 ;
LÚCIA ALVES DE OLIVEIRA FRAGA 2 ; LUIZ COSME COTTA MALAQUIAS 2,6 ;
ELAINE SPEZIALI DE FARIA 2 ; OLINDO ASSIS MARTINS-FILHO 3 ; ANA
MARIA CAETANO DE FARIA 1; RODRIGO CORREA-OLIVEIRA 3 ; ANDRÉA
GAZZINELLI 5 ; ANDRÉA TEIXEIRA CARVALHO 3 E ALDA MARIA SOARES
SILVEIRA 2
1 Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG,
Brasil
2 Universidade Vale do Rio Doce, UNIVALE, Governador Valadares, MG, Brasil
3 Centro de Pesquisa René Rachou - Fundação Oswaldo Cruz, Belo Horizonte,
MG, Brasil
4 Santa Casa de Misericórdia, Belo Horizonte, MG, Brasil
5 Escola de Enfermagem, UFMG, Belo Horizonte, MG, Brasil
6 Universidade Federal de Alfenas, Alfenas, MG, Brasil
Keywords: Schistosomiasis, Periportal fibrosis, Treatment, Cytokines
Introduction: The cytokine response to S. mansoni antigens seems to play an
important role in pathogenesis of the periportal fibrosis associated with human
schistosomiasis. The aim of this study was to investigate whether the
cytokine/chemokine pattern produced by peripheral blood mononuclear cells
upon in vitro S. mansoni antigen stimulation could be used as a biomarker of
periportal fibrosis in schistosomiasis patients and evaluate the impact of
Praziquantel treatment in this complex cytokine network. Methods: Thirty one
volunteers living in an endemic area were classified into sub-groups according
to the presence or absence of fibrosis before (FIB and non-FIB) and after
treatment (FIBT and non-FIBT) Cytokine/chemokine pattern (IFN-, TNF-α, IL-4,
IL-5, IL-13, IL-10, IL-17, TGF-β, CCL3/MIP-1α, CCL2/MCP-1 e RANTES) was
measured in PBMC culture supernatants using Cytometric Bead Array (CBA).
Cytokine/chemokine signatures were analyzed using the concept of low and
high-cytokine producers. Results: Our results demonstrate that FIB presented
a decreased cytokine production compared to non-FIB individuals. Furthermore,