immunology of infectious and parasitic diseases - XXXVII Congress ...

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higher production of TNF-, IL-10 and IL-4. In the study the fractions below 10 and 10-50 kDa did not induce in vitro production of IFN-g and IL-4. The fraction between 50-100 kDa stimulated splenocytes to produce TNF- and the fraction above 100 kDa mainly stimulated the production of IL-10. Conclusion: It is probable that the virulent strain of this microorganism induces the production of IL-4 as an immune escape mechanism. Financial support: Labimuno and FINEP Keywords: Corynebacterium pseudotuberculosis, caseous lymphadenitis, somatic antigen fractions.
Critical motifs of the Anaplasma marginale MSP1a functional epitope protect mice by balancing humoral and cellular immune response PAULA DE SOUZA SANTOS (1); ANGELA APARECIDA SERVINO DE SENA (1); RAFAEL NASCIMENTO (1); THAISE GONÇALVES ARAÚJO (1); MIRIAN MACHADO MENDES (1); TIAGO WILSON PATRIARCA MINEO (1); LUIZ RICARDO GOULART (1) (1). Federal University of Uberlândia Introduction: Bovine anaplasmosis is a hemoparasitic disease that causes considerable economic loss to the dairy and beef industries. Cattle immunized with the Anaplasma marginale MSP1 outer membrane protein complex present a protective humoral immune response; however, the efficacy of these vaccines is variable. The immunodominance of epitopes seems to be a key-limiting factor for the adaptive immunity. We have successfully demonstrated that a critical motif of the MSP1a functional epitope is essential for antibody recognition of infected animal sera (PLoS One 7(3):e33045, 2012) , but its potential to induce protective immunity is yet to be tested. Methods and Results: We have evaluated two synthetic vaccine formulations (Am1 and Am2) against A. marginale, using epitope-based approach. The experiments were carried out in murine model, which were immunized with peptides conjugated to bovine serum albumin in three 15-day interval intraperitoneal injections before challenge with live bacteria. Blood samples were analyzed for the presence of specific IgG antibodies, along with IgG2a and IgG1 antibodies, as well as for the rickettsemia analysis. Additionally, a panel containing the transcription of innate and adaptive immune responses‟ cytokines was carried out through qPCR. After challenge with A. marginale, it was observed that mice immunized with synthetic peptides presented stable body weight and no mortality, along with reduced number of infected erythrocytes (75%). In addition, the immunization process induced significantly higher production of specific IgG2a than IgG1 antibodies, along with an increased in situ expression of pro-inflammatory cytokines. Conclusion: we have demonstrated that the two synthetic peptides obtained from critical motifs within the MSP1a functional epitope were able to induce an effective immune response in mice, associated with the upregulation of effector functions of humoral and cellular immune responses, reinforcing the use of synthetic epitopes as vaccine targets against bovine anaplasmosis, aiming bacterial clearance and diminished pathogenesis. Financial Support: CNPq, FAPEMIG and CAPES.
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IMMUNOLOGY OF INFECTIOUS AND PARASI
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profiles appear to be dependent on
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INVOLVEMENT OF TNF RECEPTORS IN APO
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Effects of bioactive Cryptococcus n
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DCs expressing Indoleamine 2,3-diox
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DETECTION OF GITR ON PATIENT CERVIX
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DETECTION OF GITR AND IL-10 ON CERV
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VACCINATION WITH SM10.3 ANTIGEN, A
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Financial support: FAPESP, CAPES an
Page 19 and 20: clearance that follows Sylvio X10/4
Page 21 and 22: EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
Page 23 and 24: THERAPY WITH SCFV TRANSFECTED-DENDR
Page 25 and 26: etween the groups. The results show
Page 27 and 28: Financial support: FIOCRUZ, CNPq.
Page 29 and 30: elease of NETs from human neutrophi
Page 31 and 32: ROLE OF CD18 IN ACTIVATION OF M1 MA
Page 33 and 34: IDENTIFICATION, PURIFICATION AND CH
Page 35 and 36: with hemin showed higher levels of
Page 37 and 38: SEPTIC ARTHRITIS TRIGGERED BY S. au
Page 39 and 40: eing higher levels induced by a vir
Page 41 and 42: parasite load skin. The TGF-β was
Page 43 and 44: cultures, both treatments decreased
Page 45 and 46: observed a tendency to lower parasi
Page 47 and 48: SERUM COMPONENTS AND MONONUCLEAR CE
Page 49 and 50: VIABILITY OF LEISHMANIA (L.) CHAGAS
Page 51 and 52: EVALUATION OF THE INTERFERENCE OF S
Page 53 and 54: IMMUNODETECTION OF THE TGF- β IN L
Page 55 and 56: DIFFERENT LEVELS OF NKT AND NK CELL
Page 57 and 58: Funding: CNPQ; FAPESPA; SESPA; UFPA
Page 59 and 60: saliva that exacerbated leishmania
Page 61 and 62: BACTERIAL FILAMENTATION: SUPER-RESI
Page 63 and 64: IMMUNOLOGICAL PARAMETERS ASSOCIATED
Page 65 and 66: supernatant. BMDC stimulation with
Page 67 and 68: such as number of eosinophils, prot
Page 69: EVALUATION OF THE PRODUCTION OF TNF
Page 73 and 74: SCHISTOSOMA SPP ANTIGENS IMPAIR THE
Page 75 and 76: DOWN-REGULATION OF IFN-g RECEPTOR A
Page 77 and 78: MONOCYTES SUBSETS IN SCHISTOSOMIASI
Page 79 and 80: THE IN VITRO ANALYSIS OF iNOS-KO MO
Page 81 and 82: CCR7 IS CRITICAL FOR RESISTANCE AGA
Page 83 and 84: EVALUATION OF THE IMMUNE RESPONSE A
Page 85 and 86: our data suggest that monocyte secr
Page 87 and 88: DIVERGENT OUTCOMES OF THE INTERACTI
Page 89 and 90: they can deactivate immune effector
Page 91 and 92: Conclusions: We observed a signific
Page 93 and 94: TLR2 AND TLR4 EXPRESSION AND NUTRIT
Page 95 and 96: DEVELOPMENT OF MURINE PLACENTAL MAL
Page 97 and 98: INDUCTION OF TH17 LYMPHOCYTES CONTR
Page 99 and 100: Leishmania amazonensis INCREASES EC
Page 101 and 102: TYPE II GRANULOMA INDUCED BY SCHIST
Page 103 and 104: The Role of Eosinophils in Aspergil
Page 105 and 106: compared to baseline (p
Page 107 and 108: disordered loop and NcSAG2A present
Page 109 and 110: Conclusion: We demonstrate the exis
Page 111 and 112: WT. By contrast, uninfected AhR -/-
Page 113 and 114: in the synthesis of proinflammatory
Page 115 and 116: PRODUCTION AND EVALUATION OF CHICKE
Page 117 and 118: allow a conclusion about the differ
Page 119 and 120: contributes to parasite control and
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Financial support: FAPESP, CNPq, an
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MILTEFOSINE EXERTS ITS LEISHMANICID
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IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
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THE ROLE OF REACTIVE OXIGEN SPECIES
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INNATE AND ADAPTIVE IMMUNE REPONSE
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KINETIC OF THE SPECIFIC HUMMORAL IM
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PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
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COMPARISON OF VIRULENCE AND IMMUNE
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THE IMPACT OF TUBERCULOSIS IN THE I
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GALACTOMANNAN ANTIGENEMIA SCREENING
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NATURAL ISOTYPIC RESPONSE AGAINST P
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Characterization of costimulatory m
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P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
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OspC1 AND OspF: VIRULENCE FACTORS I
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Conclusion: Taken together, these r
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FIB produced higher proportion of f
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MØ phenotype M2, resulting in decr
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Financial support FAPESP, CAPES, CN
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ALTERATION IN THE DISTRIBUTION OF P
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BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
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FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
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NEUTROPHILS AND MACROPHAGES ARE INV
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EXPLORING THE INFLAMMATORY ROLE OF
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CERVICAL LEVELS OF INTERLEUKIN-1 BE
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Title: IDENTIFICATION BY PHAGE DISP
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PROFILE OF PRO- AND ANTI-INFLAMMATO
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IMMUNOMODULATORY MECHANISMS OF SOLU
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EVALUATION OF THE TH1, TH2 AND TH17
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production, as the blockade of PD-L
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CHARACTERIZATION OF POLYMORPHISM TN
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seems to be dependent on M. leprae
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7 with tendency to normalization on
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CHARACTERIZATION OF THE CONSERVATIO
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ASC INFLAMMASOME IS NECESSARY TO AC
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CASPASE-1 IS REQUIRED TO CONTROL OF
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The role of CD4 + T lymphocytes dur
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CRITICAL ROLE OF MYD88 EXPRESSION I
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on ROS, cathepsin B and Syk kinase
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Financial Support: FAPESP and CNPq.
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80% in symptomatic group; 45% in as
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of cells undergoing apoptosis in DT
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Financial support: FAPESP/LIM-56/HC
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identify the active components and
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EVALUATION OF HUMORAL AND CELULLAR
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Financial support: CNPq-PIBIC, FAPE
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addition, significant expression of
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THE ROLE OF K + TRANSPORTERS IN THE
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Conclusion: In this study, we estab
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Heme activates oxidative mechanisms
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ROLE OF ADENOSINE AND PROSTAGLANDIN
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Taken together, our results indicat
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Conclusion: Our findings suggest th
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Financial support: CNPq, CAPES, IOC
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number of circulating parasites in
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CRITICAL ROLE OF IL-6 IN REGULATORY
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PPAR-α AGONIST GEMFIBROZIL DECREAS
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Cutaneous Leishmaniasis in Amazonas
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CYTOTOXICITY IN IMMUNOPATHOGENESIS
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ROS production and TLR2 and 4 expre
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ROLE OF IL-4 IN THE INTESTINAL INFL
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In vitro CULTURE SYSTEM FOR Plasmod
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interactions, Tandem Affinity Purif
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in the course of human infection wi
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findings suggest that IL-17 and IL-
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23 contribute to immunological cont
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not produced during infection by C.
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Conclusion: The production of cytok
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CONCLUSION: It is likely, in this p
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expression in CCC myocardium was 64
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that mononuclear cells derived from
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Conclusion: Altogether, our data sh
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PARACOCCIDIOIDES BRASILIENSIS INHIB
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CANINE VISCERAL LEISHMANIASIS AND S
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IMMUNOMODULATORY EFFECTS OF BIOTHER
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Leishmania EFFECT ON HEME CYTOTOXIC
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ROLE OF STRONGYLOIDES VENEZUELENSIS
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TREATMENT WITH Harpagophytum procum
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