immunology of infectious and parasitic diseases - XXXVII Congress ...

CHARACTERIZATION OF POLYMORPHISM TNF-α -308 G/A IN PATIENTS WITH
CHRONIC HEPATITIS C.
SORIANE DE SOUZA CRUZ 1,2 ANDREA MONTEIRO TARRAGÔ 1,2 , FLAMIR
VICTÓRIA 3 , MARILU VICTÓRIA 3 , AYA SADAHIRO 1,3 , ADRIANA MALHEIRO 1,2
(1) Universidade Federal do Amazonas (2) Fundação de Hematologia e Hemoterapia
do Amazonas (3) Fundação de Medicina Tropical do Amazonas (4) Instituto Nacional
de Pesquisas da Amazônia
Introduction: The elimination or persistent infection by Hepatitis C virus (HCV) is
associated with viral and host factors, as well as polymorphisms cytokine gene. The
TNF-α cytokine is present in the beginning of the immune response against the virus
C. The TNF-α gene is located on human chromosome 6p21.3, where several
polymorphisms were identified and considered to affect the production of cytokine.
The aim of this study was to characterize the polymorphism -308 G/A TNFα in
patients HCV + . Methods and results: The samples were collected from 22 patients
HCV + untreated with interferon and ribavirina at the Fundação de Medicina Tropical
of Amazonas. The DNA was extracted according to the Brazol protocol followed by
PCR amplification of the DNA fragment, subsequently visualized on agarose gel
stained with 2.5% DNA Knowing Safe. The data distribution of the -308 G/A TNFα
demonstrated that 22 patients infected with HCV, 16 (72.2%) had GG genotype, of
which 02 (12.5%) were diagnosed with liver cirrhosis, while 06 (27.3 %) were GA
genotype and they had no diagnosis for liver cirrhosis. Conclusions: The data
suggest an association between liver cirrhosis patients and 308 G/GTNF-α genotype,
because it was more prevalent in this population.
Financial Support: FAPEAM, CNPq, CAPES