immunology of infectious and parasitic diseases - XXXVII Congress ...

DENDRITIC CELLS PROFILE INDUCED BY SCHISTOSOMA MANSONI
ANTIGEN IN CUTANEOUS LEISHMANIASIS PATIENTS
DIEGO MOTA LOPES (1) ; LUCIANA SANTOS CARDOSO (1,2,3) ; JAMILLE
SOUZA FERNANDES (1) ; EDGAR M. CARVALHO (1,2,4) ; MARIA ILMA
ARAUJO (1,2,4) .
(1) Immunology Service, HUPES,UFBA, Salvador; (2) National Institute of Science
and Technology in Tropical Diseases (INCT-DT)-CNPQ/MCT; (3) Department of
Life Sciences, UNEB, Salvador, Bahia; (4) Bahiana School of Medicine and
Public Health, Salvador, Bahia, Brazil.
Introduction: The Th1-immune response is associated with tissue injury in
human cutaneous leishmaniasis (CL). On the other hand, the immune response
induced by some Schistosoma mansoni antigens is able to down-regulate the
inflammatory response in immune-mediated diseases. The aim of this study
was to evaluate the potential of the S. mansoni antigen Sm29 in down-modulate
the response of monocyte-derived dendritic cells (MoDCs) from individuals with
CL stimulated in vitro with L. braziliensis soluble antigen (SLA).
Methods and Results: Twenty patients with cutaneous leishmaniasis were
enrolled in this study so far PBMC were obtained using a Ficoll-Hypaque
gradient. Monocytes were cultured in the presence of GM-CSF and IL-4 for
differentiation into dendritic cells (DCs), and then stimulated with the soluble
leishmania antigen (SLA) in the presence or absence of the S.mansoni antigen
Sm29. The expression of surface molecules HLA-DR, CD80 and CD86 on
MoDCs and the expression of cytokines such as IL-12 and TNF- and IL-10
receptor (IL-10R) by these cells have been evaluated by flow cytometry. The
study was approved by the Ethical Committee of the State University of Bahia
(UNEB). We observed that the addition of Sm29 to the MoDCs cultures
stimulated with SLA resulted in higher mean fluorescence intensity (MIF) of
CD80 (716±721) compared to cells stimulated with SLA alone (281±100;
p