immunology of infectious and parasitic diseases - XXXVII Congress ...

MODULATION OF T AND B CELL TLR EXPRESSION DURING TICK
INFESTATION
ELEN ANATRIELLO 1 ; LAUREN CRISTINA RIBEIRO 2 ; MANUELA SALES LIMA
NASCIMENTO 2 ; LAIS AMORIM SACRAMENTO 2 ; CARLO JOSÉ OLIVEIRA 3 ;
MICHELLE CHISTIANE RODRIGUES BARBOSA 1 ; JOÃO SANTANA DA
SILVA 2 ; ISABEL KINNEY FERREIRA DE MIRANDA SANTOS 2 ; BEATRIZ
ROSSETTI FERREIRA 1 .
1 School of Nursing, University of São Paulo, Ribeirão Preto, Brazil
2 School of Medicine, University of São Paulo, Ribeirão Preto, Brazil.
3 Institute of Biological and Natural Sciences, Federal University of Triângulo
Mineiro –
UFTM, Uberaba, Brazil. 3
Introdution: The brown dog tick, Rhipicephalus sanguineus, is found
worldwide, and is one of the most important vectors of diseases to dogs. In
order to feed with success, ticks inoculate saliva that modulates both, innate
and acquired immune responses of their hosts. Our group has shown that mice
infested with R. sanguineus ticks develop a Th2 type immune response, and
that tick saliva modulates the function of dendritic cells (DCs) stimulated by Toll
like receptors (TLRs) ligands. We also observed that tick saliva induces in vitro
a high expression of TLR2 on the DCs surface. These results suggest that
saliva may modulate the host immune response via TLR impairment in diverse
cell kinds. Objectives: The present work evaluates the role of tick infestation on
the expression of TLRs molecules on cells from the acquired immune response.
Methods and Results: We examined the percentage of T and B spleen cells
expressing TLR1, 2, 4, 5, 6 and 9 from twice-tick-infested C57Bl/6 mice
(n=4/group, 6 ticks/mice) using flow cytometry. The results showed that T and B
cells expressing TLR4 were diminished (22 and 28% of reduction, respectively,
p