immunology of infectious and parasitic diseases - XXXVII Congress ...

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alveolar macrophages was observed in TNFR1 -/- mice at day 3 after BCG infection. Surface expression of TNFR2 in C57Bl/6 mice was lower on days 3 and 7, which was accompanied by increased levels of sTNFR2 in mice infected with both strains of M. bovis. Conclusions: These results suggest a potential role for TNFR1 in mycobacteria induced alveolar macrophage apoptosis in vivo. In this scenario, shedding of TNFR1 seems to contribute to the modulation of macrophage apoptosis in a strain dependent manner. Financial support: FAPEMIG, CNPq and CAPES.
Effects of bioactive Cryptococcus neoformans mimotopes on vascular endothelial growth fator, nitric oxide and chemokine production in vitro by immune cells. SILVA, P.R. (PG) 1,2 ; BOMBANA, H.S. (IC) 2 ; ARAÚJO, A.S. (PG) 2 ; NOGUEIRA, M.R. (PG) 2 ; GARCIA, F.G. (PG) 2 ; OLIVEIRA, A.C.S. (PG) 2 ; SILVA-TEIXEIRA, D.N. 2 1 2 Centro de Formação Especial/CEFORES-UFTM, Laboratório de Imunobiologia e Pesquisa em Ativação Celular (LIPAC), Instituto de Ciências da Saúde (ICS), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG. e-mail: silvateixeira@mednet.com.br Key words: Cryptococcus neoformans, “phage display”, mimotopes, VEGF, nitric oxide (NO). Introduction and objectives: Cryptococcus neoformans infected patients show an augmented permeability of blood-brain barrier, which contributes to increase morbidity and mortality in cryptococcosis. C. neoformans access to central nervous system (CNS) is facilitated by vasoactive mediators produced by immune cells, such as nitric oxide (NO), vascular endothelial growth factor (VEGF), some cytokines and chemokines. In the present work we investigated the effects of C. neoformans mimetic peptides (mimotopes) obtained by “phage display” on in vitro production of vasoactive mediators (VEGF, NO, IL-1b, CCL- 2 and CXCL-8) by human mononuclear cells (PBMC) and polymorphonuclear leukocytes (PMN). Methods: PBMC and PMN from healthy volunteers (n=12) were isolated by a double density ficoll-hypaque gradient. Mimotopes were selected by “phage display” with a monoclonal antibody (18b7 Mab) against C. neoformans capsular glucoronoxylomanan (GXM). After a chemical synthesis, peptides were validated by ELISA. A linear sequence peptide (PL), a conformational peptide containing one disulfide bridge (PC) and a double conformational peptide (PD), made of two PC peptides linked by a GGGS spacer sequence, were used to stimulate cells in vitro. VEGF, IL-1b, CCL-2 and CXCL-8
Page 1 and 2: IMMUNOLOGY OF INFECTIOUS AND PARASI
Page 3 and 4: profiles appear to be dependent on
Page 5: INVOLVEMENT OF TNF RECEPTORS IN APO
Page 9 and 10: DCs expressing Indoleamine 2,3-diox
Page 11 and 12: DETECTION OF GITR ON PATIENT CERVIX
Page 13 and 14: DETECTION OF GITR AND IL-10 ON CERV
Page 15 and 16: VACCINATION WITH SM10.3 ANTIGEN, A
Page 17 and 18: Financial support: FAPESP, CAPES an
Page 19 and 20: clearance that follows Sylvio X10/4
Page 21 and 22: EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
Page 23 and 24: THERAPY WITH SCFV TRANSFECTED-DENDR
Page 25 and 26: etween the groups. The results show
Page 27 and 28: Financial support: FIOCRUZ, CNPq.
Page 29 and 30: elease of NETs from human neutrophi
Page 31 and 32: ROLE OF CD18 IN ACTIVATION OF M1 MA
Page 33 and 34: IDENTIFICATION, PURIFICATION AND CH
Page 35 and 36: with hemin showed higher levels of
Page 37 and 38: SEPTIC ARTHRITIS TRIGGERED BY S. au
Page 39 and 40: eing higher levels induced by a vir
Page 41 and 42: parasite load skin. The TGF-β was
Page 43 and 44: cultures, both treatments decreased
Page 45 and 46: observed a tendency to lower parasi
Page 47 and 48: SERUM COMPONENTS AND MONONUCLEAR CE
Page 49 and 50: VIABILITY OF LEISHMANIA (L.) CHAGAS
Page 51 and 52: EVALUATION OF THE INTERFERENCE OF S
Page 53 and 54: IMMUNODETECTION OF THE TGF- β IN L
Page 55 and 56: DIFFERENT LEVELS OF NKT AND NK CELL
Page 57 and 58:
Funding: CNPQ; FAPESPA; SESPA; UFPA
Page 59 and 60:
saliva that exacerbated leishmania
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BACTERIAL FILAMENTATION: SUPER-RESI
Page 63 and 64:
IMMUNOLOGICAL PARAMETERS ASSOCIATED
Page 65 and 66:
supernatant. BMDC stimulation with
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such as number of eosinophils, prot
Page 69 and 70:
EVALUATION OF THE PRODUCTION OF TNF
Page 71 and 72:
Critical motifs of the Anaplasma ma
Page 73 and 74:
SCHISTOSOMA SPP ANTIGENS IMPAIR THE
Page 75 and 76:
DOWN-REGULATION OF IFN-g RECEPTOR A
Page 77 and 78:
MONOCYTES SUBSETS IN SCHISTOSOMIASI
Page 79 and 80:
THE IN VITRO ANALYSIS OF iNOS-KO MO
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CCR7 IS CRITICAL FOR RESISTANCE AGA
Page 83 and 84:
EVALUATION OF THE IMMUNE RESPONSE A
Page 85 and 86:
our data suggest that monocyte secr
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DIVERGENT OUTCOMES OF THE INTERACTI
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they can deactivate immune effector
Page 91 and 92:
Conclusions: We observed a signific
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TLR2 AND TLR4 EXPRESSION AND NUTRIT
Page 95 and 96:
DEVELOPMENT OF MURINE PLACENTAL MAL
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INDUCTION OF TH17 LYMPHOCYTES CONTR
Page 99 and 100:
Leishmania amazonensis INCREASES EC
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TYPE II GRANULOMA INDUCED BY SCHIST
Page 103 and 104:
The Role of Eosinophils in Aspergil
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compared to baseline (p
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disordered loop and NcSAG2A present
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Conclusion: We demonstrate the exis
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WT. By contrast, uninfected AhR -/-
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in the synthesis of proinflammatory
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PRODUCTION AND EVALUATION OF CHICKE
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allow a conclusion about the differ
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contributes to parasite control and
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Financial support: FAPESP, CNPq, an
Page 123 and 124:
MILTEFOSINE EXERTS ITS LEISHMANICID
Page 125 and 126:
IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
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THE ROLE OF REACTIVE OXIGEN SPECIES
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INNATE AND ADAPTIVE IMMUNE REPONSE
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KINETIC OF THE SPECIFIC HUMMORAL IM
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PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
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COMPARISON OF VIRULENCE AND IMMUNE
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THE IMPACT OF TUBERCULOSIS IN THE I
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GALACTOMANNAN ANTIGENEMIA SCREENING
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NATURAL ISOTYPIC RESPONSE AGAINST P
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Characterization of costimulatory m
Page 151 and 152:
P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
Page 163 and 164:
OspC1 AND OspF: VIRULENCE FACTORS I
Page 165 and 166:
Conclusion: Taken together, these r
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FIB produced higher proportion of f
Page 169 and 170:
MØ phenotype M2, resulting in decr
Page 171 and 172:
Financial support FAPESP, CAPES, CN
Page 173 and 174:
ALTERATION IN THE DISTRIBUTION OF P
Page 175 and 176:
BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
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FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
Page 183 and 184:
NEUTROPHILS AND MACROPHAGES ARE INV
Page 185 and 186:
EXPLORING THE INFLAMMATORY ROLE OF
Page 187 and 188:
CERVICAL LEVELS OF INTERLEUKIN-1 BE
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Title: IDENTIFICATION BY PHAGE DISP
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PROFILE OF PRO- AND ANTI-INFLAMMATO
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IMMUNOMODULATORY MECHANISMS OF SOLU
Page 195 and 196:
EVALUATION OF THE TH1, TH2 AND TH17
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production, as the blockade of PD-L
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CHARACTERIZATION OF POLYMORPHISM TN
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seems to be dependent on M. leprae
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7 with tendency to normalization on
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CHARACTERIZATION OF THE CONSERVATIO
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ASC INFLAMMASOME IS NECESSARY TO AC
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CASPASE-1 IS REQUIRED TO CONTROL OF
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The role of CD4 + T lymphocytes dur
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CRITICAL ROLE OF MYD88 EXPRESSION I
Page 215 and 216:
on ROS, cathepsin B and Syk kinase
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Financial Support: FAPESP and CNPq.
Page 219 and 220:
80% in symptomatic group; 45% in as
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of cells undergoing apoptosis in DT
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Financial support: FAPESP/LIM-56/HC
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identify the active components and
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EVALUATION OF HUMORAL AND CELULLAR
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Financial support: CNPq-PIBIC, FAPE
Page 231 and 232:
addition, significant expression of
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THE ROLE OF K + TRANSPORTERS IN THE
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Conclusion: In this study, we estab
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Heme activates oxidative mechanisms
Page 239 and 240:
ROLE OF ADENOSINE AND PROSTAGLANDIN
Page 241 and 242:
Taken together, our results indicat
Page 243 and 244:
Conclusion: Our findings suggest th
Page 245 and 246:
Financial support: CNPq, CAPES, IOC
Page 247 and 248:
number of circulating parasites in
Page 249 and 250:
CRITICAL ROLE OF IL-6 IN REGULATORY
Page 251 and 252:
PPAR-α AGONIST GEMFIBROZIL DECREAS
Page 253 and 254:
Cutaneous Leishmaniasis in Amazonas
Page 255 and 256:
CYTOTOXICITY IN IMMUNOPATHOGENESIS
Page 257 and 258:
ROS production and TLR2 and 4 expre
Page 259 and 260:
ROLE OF IL-4 IN THE INTESTINAL INFL
Page 261 and 262:
In vitro CULTURE SYSTEM FOR Plasmod
Page 263 and 264:
interactions, Tandem Affinity Purif
Page 265 and 266:
in the course of human infection wi
Page 267 and 268:
findings suggest that IL-17 and IL-
Page 269 and 270:
23 contribute to immunological cont
Page 271 and 272:
not produced during infection by C.
Page 273 and 274:
Conclusion: The production of cytok
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CONCLUSION: It is likely, in this p
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expression in CCC myocardium was 64
Page 279 and 280:
that mononuclear cells derived from
Page 281 and 282:
Conclusion: Altogether, our data sh
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PARACOCCIDIOIDES BRASILIENSIS INHIB
Page 285 and 286:
CANINE VISCERAL LEISHMANIASIS AND S
Page 287 and 288:
IMMUNOMODULATORY EFFECTS OF BIOTHER
Page 289 and 290:
Leishmania EFFECT ON HEME CYTOTOXIC
Page 291 and 292:
ROLE OF STRONGYLOIDES VENEZUELENSIS
Page 293 and 294:
TREATMENT WITH Harpagophytum procum
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